Home > Courses > Biotechnology > BIO203

BIO203 : Methods in Molecular Biology

I like this Course

Course Info

Course Category

Biotechnology

Course Level

Undergraduate

Credit Hours

3

Pre-requisites

N/A

Instructor

Dr. Saeeda Kalsoom
Ph.D
University of Veterinary and Animal Sciences, (UVAS) Lahore.

Course Contents

COURSE CONTENTS:

Theory:

Polymerase chain reaction, Steps of PCR, Ingredients of PCR, Primers for PCR, DNA polymerases, Standard PCR reaction, Interpretation of results, Types of PCR, Nested PCR, Multiplex PCR, Reverse transcriptase PCR, Real time PCR, Hot start PCR, Asymmetric PCR, Long PCR, Allelic specific PCR , Colony PCR, In situ PCR, Inverse PCR, AFLP PCR, Assembly PCR, Suicide PCR, Methylation specific PCR, Inter sequence specific PCR, Ligation mediated PCR, Whole genome amplification PCR, Mini primer PCR, Advantages and limitations of PCR, Applications of PCR, PCR-Medicine, PCR-Forensic sciences, PCR-Agricultural sciences and environment, PCR-Molecular paleontology, Types of restriction and modification (R-M) system, Nomenclature of restriction endonucleases ,Target sites of restriction endonucleases, Number and size of restriction fragments, Summary of restriction endonucleases, DNA modifying enzymes, Methods of joining DNA fragments, DNA ligase to create covalent recombinant DNA, Alkaline Phosphatase, Blunt end ligation via linker molecules, Adaptors, Southern blotting, Southern blotting procedure, Southern blotting probes, Transfer methods in southern blotting, Mutations identification by southern blotting, Northern blotting, Northern blot applications, Western blotting, Western blotting procedure 

Practicals:

1.   

1

PCR (types of PCR)

2

Types of Gel Electrophoresis

3

Recombinant DNA technology

4

RT-qPCR (genotyping)

5

RT-qPCR (gene expression)

6

RT-qPCR (application)

7

RT-qPCR (Chemistries)

8

RT-qPCR (prob designing)

9

RFLP

10

ARMS Technoque

11

STR technique

12

Sequencing Technologies

13

Genotyping by sequencing

14

Phylogenetic analysis

15

genotyping by sequencing

16

Bioinformatics software and tools