Home > Courses > Biotechnology > BIO203

BIO203 : Methods in Molecular Biology

I like this Course

Course Info

Course Category

Biotechnology

Course Level

Undergraduate

Credit Hours

3

Pre-requisites

N/A

Instructor

Ms Saima Mustafa
Ph.D Scholar
Bahaudin Zakarya University, Multan

Course Contents

COURSE CONTENTS:

Theory:

Introduction to recombinant DNA technology; restriction and modifying enzymes; cloning and expression vectors and their types; expression of recombinant proteins and their purification by affinity chromatography; polymerase chain reaction (PCR) - types; (inverse, touch-down, nested, hemi-nested, pit stop, multiplex, reverse transcriptase, RACE, real-time) and its applications; detection of mutations and/or SNPs; DNA fingerprinting; analysis of nucleic acids by gel electrophoresis – horizontal, vertical, pulse field, denaturing gradient gel electrophoresis; analysis of proteins by native and SDS-PAGE; 2-D gels; generation of antibodies and their uses; enzymelinked immunosorbant assay; Southern, Western, Northern blotting.

Practical:

1.   

1

PCR (types of PCR)

2

Types of Gel Electrophoresis

3

Recombinant DNA technology

4

RT-qPCR (genotyping)

5

RT-qPCR (gene expression)

6

RT-qPCR (application)

7

RT-qPCR (Chemistries)

8

RT-qPCR (prob designing)

9

RFLP

10

ARMS Technoque

11

STR technique

12

Sequencing Technologies

13

Genotyping by sequencing

14

Phylogenetic analysis

15

genotyping by sequencing

16

Bioinformatics software and tools