COURSE CONTENTS:
Theory:
Introduction to recombinant DNA technology;
restriction and modifying enzymes; cloning and expression vectors and their
types; expression of recombinant proteins and their purification by affinity
chromatography; polymerase chain reaction (PCR) - types; (inverse, touch-down,
nested, hemi-nested, pit stop, multiplex, reverse transcriptase, RACE,
real-time) and its applications; detection of mutations and/or SNPs; DNA
fingerprinting; analysis of nucleic acids by gel electrophoresis – horizontal,
vertical, pulse field, denaturing gradient gel electrophoresis; analysis of
proteins by native and SDS-PAGE; 2-D gels; generation of antibodies and their
uses; enzymelinked immunosorbant assay; Southern, Western, Northern blotting.
Practical:1.
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1
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PCR (types of PCR)
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2
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Types of Gel Electrophoresis
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3
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Recombinant DNA technology
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4
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RT-qPCR (genotyping)
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5
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RT-qPCR (gene expression)
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6
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RT-qPCR (application)
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7
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RT-qPCR (Chemistries)
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8
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RT-qPCR (prob designing)
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9
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RFLP
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10
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ARMS Technoque
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11
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STR technique
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12
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Sequencing Technologies
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13
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Genotyping by sequencing
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14
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Phylogenetic analysis
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15
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genotyping by sequencing
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16
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Bioinformatics software and tools
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