Polymerase chain reaction,
Steps of PCR, Ingredients of PCR, Primers for PCR, DNA polymerases, Standard
PCR reaction, Interpretation of results, Types of PCR, Nested PCR, Multiplex
PCR, Reverse transcriptase PCR, Real time PCR, Hot start PCR, Asymmetric PCR, Long
PCR, Allelic specific PCR , Colony PCR, In situ PCR, Inverse PCR, AFLP
PCR, Assembly PCR, Suicide PCR, Methylation specific PCR, Inter sequence
specific PCR, Ligation mediated PCR, Whole genome amplification PCR, Mini
primer PCR, Advantages and limitations of PCR, Applications of PCR, PCR-Medicine,
PCR-Forensic sciences, PCR-Agricultural sciences and environment, PCR-Molecular
paleontology, Types of restriction and modification (R-M) system, Nomenclature
of restriction endonucleases ,Target sites of restriction endonucleases, Number
and size of restriction fragments, Summary of restriction endonucleases, DNA
modifying enzymes, Methods of joining DNA fragments, DNA ligase to create
covalent recombinant DNA, Alkaline Phosphatase, Blunt end ligation via linker
molecules, Adaptors, Southern blotting, Southern
blotting procedure, Southern blotting probes, Transfer methods in southern
blotting, Mutations identification by southern blotting, Northern blotting, Northern
blot applications, Western blotting, Western blotting procedure
Practicals:
1.
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1
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PCR (types of PCR)
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2
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Types of Gel Electrophoresis
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3
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Recombinant DNA technology
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4
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RT-qPCR (genotyping)
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5
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RT-qPCR (gene expression)
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6
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RT-qPCR (application)
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7
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RT-qPCR (Chemistries)
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8
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RT-qPCR (prob designing)
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9
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RFLP
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10
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ARMS Technoque
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11
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STR technique
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12
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Sequencing Technologies
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13
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Genotyping by sequencing
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14
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Phylogenetic analysis
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15
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genotyping by sequencing
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16
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Bioinformatics software and tools
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