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BIO204 : Principles of Biochemical Engineering

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Course Info

Course Category

Biotechnology

Course Level

Undergraduate

Credit Hours

3

Pre-requisites

N/A

Instructor

Dr. M. Mohsin Javed
Ph.D., Post. Doc. (USA)
USA

Course Contents

Course Contents

Theory: Introduction to microorganisms and biological molecules; principles of enzyme catalysis; methods of enzyme and cell immobilization; enzyme kinetics; internal mass transfer effect on immobilized growth; stoichiometry models of microbial growth; structured model, of microbial growth; bioreactors - continuous stirred tank bioreactors, plug-flow and packed bed bioreactors, imperfect mixing, fed batch bioreactors, gas liquid mass transfer in bioreactors, power requirement for bioreactor, sterilization and heat transfer in bioreactors; introduction to bioproduct recovery; biological product manufacturing; economic analysis of bioprocesses; case study: penicillin.

Practical:

1. Initiation of a plant or animal cell/tissue culture in a simple conical flask or in a fermenter
2. Initiation of a bacterial culture in a shaking incubator 
3. Initiation of a bacterial culture in a fermenter
4. Sterilization through moist heating
5. Sterilization through Dry heat
6. Sterilization by using chemical methods
7. Sterilization by using filtration methods
8. Media formulation on base of media consistency
9. Media formulation for bacterial strain selection and differentiation

10. Growth kinetics
11. Product recovery 
14. Production of enzymes by solid state fermentation
15. Batch submerged fermentation of baker''''''''''''''''''''''''''''''''s yeast in a shaker flask
16 Batch submerged fermentation of baker''''''''''''''''''''''''''''''''s yeast in a shaker flask