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BT734 : Research Methods in Biotechnology

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Dr. Nadeem Sheikh
Ph.D. (Georg-August University, Göttingen, GERMANY)
(Georg-August University, Göttingen, GERMANY

Course Contents

Record Keeping and Safety Rules,

The Basic Skills of the Biotechnology Workplace,

Aseptic Technique and Establishing Pure Cultures: The Streak Plate and Culture Transfer, Preparation of the Culture Media,

The Growth Curve,

Isolation of Plasmid DNA from Escherichia coli: The Mini- Prep,

Purification, Concentration, and Quantitation of DNA,

Large-Scale Isolation of Plasmid DNA by Column Chromatography,

Amplification of a Lac-Z Gene Fragment by the Polymerase Chain Reaction,

Restriction Digestion,

Agarose Gel Electrophoresis,

Southern Transfer, Preparation, Purification, and Hybridization of Probe,

Transformation of Saccharomyces cerevisiae,

Isolation of Plasmid from Yeast and Escherichia coli,


Qualitative Assay for β-Galactosidase in Yeast Colonies,

Determination of β -Galactosidase in Permeabilized Yeast Cells,

Assay of β -Galactosidase in Cell Extracts,

β-Galactosidase Purification, Ammonium sulfate salting-out of β-galactosidase and column chromatography,

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis,

Western Blot: Probe of Protein Blot with Antibody to β Galactosidase,

DNA Sequencing