Course Overview
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Course Synopsis
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This is one of the course which introduces students to the fundamentals of genetic science through a basic study of the genetic material, what is the mode of inheritance, genetic and inherited diseases, concept of classical genetics, long way of Mendelian genetics to modern genetics, speciation, and evolutionary genetics, differentiation between microevolution and macroevolution. Moreover, advanced studies of genes, genomes and molecular genetics will also be touches in the later part of the course.
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Course Learning Outcomes
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Mendelian Genetics, Modern Genetics and Molecular Genetics
- classical genetics, modern genetics, types of genetics, mode of inheritances, genetics diseases, genomics, Modern research and research techniques in genetics, molecular genetics. understanding of all these topics will enable you to understand the advanced courses in general genetics, molecular biology, cell biology, recombinant DNA technology and genomics
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Course Calendar
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Week 01
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4
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Mendel s’ law of Dominance
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5
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Monohybrid Cross and Dihybrid Cross
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6
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Mendel s’ law of Segregations
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7
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Mendel s' law of Independent Assortment
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8
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Modern Genetics Concepts
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12
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Genetic and Acquired Variation
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15
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Heredity and Variation in Mendel s’ work
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Week 02
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16
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Mendelian and Non-Mendelian Inheritance
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17
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Conclusions of Mendelian Inheritance
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18
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Exceptions to Mendelian Inheritance
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26
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Cell, Nucleus and Chromosome
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27
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Chromosome Numbers in Different Species
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28
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Chromosomes in Prokaryotes and Eukaryotes
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29
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Salient features of Chromosomes
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Week 03
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34
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Euchromatin and Heterochromatin
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35
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Staining and Banding Chromosomes
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37
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Chromatin and Chromosomes
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38
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High Order Chromatin Folding
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41
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Prokaryotic Chromosomes
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42
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Sex Chromosomes and Sex determination
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43
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Salient features of DNA
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Week 04
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46
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Chargaff Rule of Base Ratio
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52
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Unreplicated and Replicated Chromosomes
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60
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Somatic cells and Gametes
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Week 05
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61
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Homologous Chromosomes
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65
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Metaphase I of Meiosis
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67
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Telophase I of Meiosis
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68
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Prophase II of Meiosis
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69
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Metaphase II of Meiosis
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70
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Anaphase II of Meiosis
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71
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Telophase II of Meiosis
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73
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Summary - Mitosis and Meiosis
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74
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Chromosomal Theory of Inheritance
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75
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Experiments of T H. Morgan
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Week 06
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76
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Effect of Linkage on Inheritance
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78
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Linkage map of Drosophila Chromosome
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79
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Chromosomal Basis of Sex Determination
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80
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Sex Determination in Other Organisms
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81
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Inheritance of Sex linked genes
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82
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Genetic Mapping in Humans
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83
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Concept of Multiple Alleles
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84
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Multiple Alleles - our blood group
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85
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Blood groups and Transfusions
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86
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Multiple alleles effect in Drosophila
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87
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Correlation of Alleles and Genotypes
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88
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Linkage and Cross over
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90
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Genetic and Physical Map
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Week 07
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91
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Mapping genes In human Pedigrees
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93
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Examples of Polygenic inheritance
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96
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Types of Epistasis-Dominant
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97
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Example of Epistasis-Labrador Retrievers
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99
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Epigenetic Modifications
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100
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Epigenetics regulated Phenomena
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101
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Therapies Targeting Epigenetics
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102
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Environmental Factors and Epigenetics
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103
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Epigenetics- Conclusions
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104
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Penetrance and Expressivity
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105
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Penetrance and Expressivity- Example
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Week 08
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106
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Chromosomal Aberrations
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107
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Karyotyping- international description
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108
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Defining Chromosome Location
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109
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Common Numerical Abnormalities
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110
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Structural Abnormalities
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117
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Polyploidy and Aneuploidy
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118
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Chromosomal Deletions
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119
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Chromosomal Insertions
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120
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Chromosomal Inversions
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Week 09
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124
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Somatic and Germline Mutation
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125
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Forward, Reverse and Neutral Mutation
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126
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Loss of function, Gain of function
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127
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Spontaneous and Induced Mutation
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Week 10
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136
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How Mutations alter Gene Function
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137
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Mutation Nomenclature
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138
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Salient feature of Mutation Nomenclature
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139
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Mutation at DNA, RNA and Protein level
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140
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Mutation in Intronic Sequence
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141
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Specific changes in Sequence
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143
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What are Genetic Disorders
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144
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Inheritance Patterns of Genetic Disorders
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145
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Autosomal Recessive Inheritance
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146
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Characteristics of Autosomal Recessive Inheritance
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147
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Autosomal Dominant Inheritance
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148
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Variation in Phenotype of A.D. inheritance
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149
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Dominance and Recessiveness
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150
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X-linked recessive inheritance
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Week 11
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151
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X-linked dominant inheritance
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152
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Maternal inheritance of mt DNA
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153
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Multifactorial inheritance
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154
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Frequency of genetic disorders
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155
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Diagnostic tests for genetic disorders
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157
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Complications to basic inheritance pattern
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161
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Types of polymorphism
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162
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Restriction fragment length polymorphism
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164
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Short tandem repeat polymorphism
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Week 12
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167
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Screening and importance of SNPs
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168
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Genetic bases Problem 1
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169
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Genetic bases Problem 2
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170
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Genetic bases Problem 3
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171
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Genetic bases Problem 4
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172
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Genetic bases Problem 5
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173
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Genetic bases Problem 6
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174
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Genetic bases Problem 7
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175
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Genetic bases Problem 8
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176
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Genetic bases Problem 9
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177
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Genetic bases Problem 10
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178
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Genetic bases Problem 11
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179
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Genetic bases Problem 12
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180
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Genetic bases Problem 13
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Week 13
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181
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Genetic bases Problem 14
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182
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Genetic bases Problem 15
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183
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Genetic bases Problem 16
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184
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Genetic bases Problem 17
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185
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Source and recovery of DNA
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186
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DNA amount for molecular techniques
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187
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Methods of DNA purification
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188
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Organic method reagents
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189
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Organic method summary
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190
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Organic method procedure (I)
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191
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Organic method procedure (II)
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192
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Phenol chloroform in organic method
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193
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Role of ethanol and isopropanol
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194
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Suspension and storage of DNA
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195
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Organic method properties
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Week 14
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196
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DNA extraction- non organic method
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198
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RNA purification- samples
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199
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RNA purification -blood
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200
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RNA purification- organic method
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201
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RNA purification- affinity method
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202
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RNA purification- human blood (I)
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203
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RNA purification - human blood (II)
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204
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RNA purification- human blood (III)
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208
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Giemsa Staining procedure
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209
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C banding of chromosomes
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210
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Q banding of chromosomes
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Week 15
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211
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R banding of chromosomes
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212
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T banding of chromosomes
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213
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Polymerase chain reaction
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218
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Standard PCR reaction
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219
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Interpretation of results
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223
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Reverse transcriptase PCR
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Week 16
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235
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Methylation specific PCR
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236
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Inter sequence specific PCR
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237
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Ligation mediated PCR
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238
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Whole genome amplification PCR
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240
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Advantages and limitations of PCR
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243
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Southern blotting procedure
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244
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Southern blotting probes
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245
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Transfer methods in southern blotting
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246
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Mutations identification by southern blotting
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248
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Northern blot applications
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250
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Western blotting procedure
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251
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Preparation of Stock and Working Solutions
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252
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Sources and Recovery of DNA
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253
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DNA Extraction Part 1
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254
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DNA Extraction Part 2
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255
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RNA Purification from Blood
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256
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Quantification of Nucleic acids by Gel electrophoresis
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257
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Quantification of Nucleic acids by Spectrophotometer
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262
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Karyotyping (Practical)
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263
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Chromosome Staining, Banding of Chromosomes (C, Q, R, T)
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