Course Overview
|
Course Synopsis
|
This course is designed for graduate students who will explore basic concepts about molecular biology topics starting from nucleic acids (DNA and RNA), genes, genome and then finally up to the gene expression level. This course will enable the students to get sufficient knowledge about the subject to carry out research in the same field.
|
Course Learning Outcomes
|
Understanding the central dogma of molecular biology
- After the successful completion of this course, students will develop basic knowledge and skills in molecular biology and become aware of the complexity and harmony of the cell. As you proceed through the modules, you will become able to apply this knowledge, skill, and awareness to topics such as: structure and functions of DNA/RNA and proteins, DNA/RNA extraction, PCR, recombinant DNA technology, blotting techniques, concept of gene and chromosomes, transcription, translation and DNA replication.
- This course is aimed to provide ample knowledge to the students which is sufficient to fulfill the requirements of their BS degree. Students will be able to develop classical understanding about molecular biology. An important objective is to provide a supportive environment for students to cultivate higher order thinking skills by synthesizing concepts and by analyzing and interpreting different questions.
|
Course Calendar
|
|
|
Week 01
|
1
|
Introduction to Molecular Biology
|
|
2
|
History of Molecular Biology
|
|
3
|
Achievements of Molecular Biology
|
|
5
|
Chemical composition of DNA
|
|
6
|
Nucleoside & Nucleotide
|
|
7
|
Types of Deoxyribonucleotides
|
|
8
|
How do Deoxyribonucleotides Join?
|
|
10
|
Work of Franklin & Wilkins (1950s)
|
|
12
|
Chemical composition of RNA
|
|
13
|
Types of Ribonucleotides
|
Week 02
|
16
|
Nature of Genetic Material
|
|
17
|
Griffith’s Experiments
|
|
18
|
Transformation Experiments
|
|
19
|
Avery, MacLeod, and McCarty’s experiment
|
|
20
|
Hershey & Chase experiment
|
|
21
|
Chemical composition of proteins
|
|
22
|
Primary structure of proteins
|
|
23
|
Secondary structure of proteins
|
|
26
|
Tertiary Structure of Proteins
|
|
27
|
Quaternary Structure of Proteins
|
Week 03
|
28
|
Genetic Materials in Viruses
|
|
29
|
Organization of Genetic Material in Bacteria -1
|
|
30
|
Organization of Genetic Material in Bacteria - 2
|
|
31
|
Organization of Genetic Material in Eukaryotes
|
Week 04
|
37
|
Experiment of Meselson & Stahl
|
|
38
|
Chemistry of DNA Synthesis - 1
|
|
39
|
Chemistry of DNA Synthesis - 2
|
|
40
|
Mechanism of DNA Polymerase
|
|
41
|
DNA Polymerases Resemble a Hand - 1
|
|
42
|
DNA Polymerases Resemble a Hand - 2
|
|
47
|
INITIATION OF REPLICATION
|
|
50
|
TYPE II TOPOISOMERASES
|
|
56
|
Cyclobutane pyrimidine dimer
|
|
58
|
x rays and gamma rays damage
|
Week 05
|
59
|
DNA instability in water
|
|
60
|
water-mediated deamination
|
|
61
|
water-mediated deamination (Conti..)
|
|
62
|
Oxidative damage to DNA
|
|
63
|
Oxidative damage to DNA (Conti..)
|
|
64
|
Alkylation damage to DNA
|
|
65
|
Alkylation damage to DNA (Conti..)
|
|
67
|
DNA damage by aflatoxins
|
|
68
|
Chemical cross-linking agents
|
|
69
|
DNA damage by Psoralen
|
|
70
|
DNA damage by Cisplatin
|
|
71
|
Base analogs and interclating agents
|
Week 06
|
72
|
Direct reversal of DNA damage
|
|
75
|
Mechanism of CPD photolyase
|
|
77
|
Damage Reversal by Dealkylation
|
Week 07
|
80
|
Base Excision Repair Pathway
|
|
83
|
Nucleotide Excision Repair
|
|
84
|
Nucleotide Excision Repair of UV-induced Damage
|
|
85
|
UvrA, UvrB, and UvrC Proteins
|
|
88
|
Mismatch Repair System in E. coli
|
|
89
|
MutS, MutL, & MutH Proteins
|
|
90
|
Mismatch Repair in Eukaryotes
|
|
91
|
Human Mismatch repair system
|
Week 08
|
93
|
The Adapter Hypothesis of Crick
|
|
94
|
Discovery of the Transfer RNA
|
|
95
|
Discovery of the messenger RNA
|
|
96
|
Synthesis of RNA upon DNA Templates
|
|
97
|
Establishing the Genetic code
|
|
100
|
Transcription Process
|
|
101
|
Elongation during transcription
|
Week 09
|
102
|
Transcription cycle in Bacteria
|
|
103
|
transition to the open step
|
|
104
|
transition to the open step 2
|
|
105
|
Initial transcription
|
|
107
|
the Elongating Polymerases
|
|
108
|
Termination of the Transcription
|
|
109
|
Termination of the Transcription II
|
|
110
|
Rho independent termination
|
|
111
|
Transcription in Eukaryotes
|
|
112
|
Formation of Pre-initiation Complex
|
|
114
|
General Transcription Factors
|
|
115
|
General Transcription Factors (Conti...)
|
|
117
|
RNA Elongation and Proofreading
|
|
118
|
RNA Elongation and Proofreading (Conti...)
|
|
119
|
Transcription Termination
|
|
120
|
Polymerases I and III
|
|
121
|
Polymerases I and III (Conti...)
|
Week 10
|
123
|
How splicing site is determined
|
|
127
|
Self-Splicing Introns
|
|
129
|
Finding the Splice Sites Correctly
|
|
130
|
Alternative Spliceosome
|
|
132
|
Alternative Splicing 2
|
|
133
|
Mutually Exclusive Splicing
|
|
134
|
Regulation by Activators and Repressors
|
|
135
|
Regulation by Activators and Repressors 2
|
|
136
|
Mechanism of RNA Editing
|
|
137
|
Mechanism of RNA Editing 2
|
|
138
|
RNA Editing by Nucleotide Deamination
|
|
139
|
RNA Editing by Nucleotide Deamination 2
|
Week 11
|
145
|
Secondary structure of tRNA
|
|
146
|
Attachment of aminoacids to tRNAs
|
|
148
|
Structure of Peptide Bonds
|
|
149
|
Binding sites on the ribosomes on tRNA
|
|
150
|
Initiation of translation
|
|
151
|
the initiation factors
|
|
152
|
Translation elongation
|
|
153
|
The Ribosome is a ribozyme
|
|
154
|
The translocation in the large subunits
|
|
155
|
Termination of the Translation
|
|
156
|
Termination of the Translation part 2
|
|
157
|
Nascent Polypeptide processing and folding
|
|
158
|
Nascent Polypeptide processing and folding part 2
|
Week 12
|
160
|
Regulation by RNAs in Bacteria
|
|
161
|
Regulation by RNAs in Bacteria part 2
|
|
164
|
Regulation by RNAs in Eukaryoutes
|
|
166
|
Enhancers and Silencers
|
|
167
|
Monocistronic or Polycistronic mRNA
|
|
168
|
mRNA has a very short life span
|
|
169
|
Controlling the rate of mRNA synthesis
|
Week 13
|
171
|
Lac structural genes are regulated
|
|
172
|
Regulation of Lac mRNA
|
|
174
|
Allolactose; the inducer of lactose operon
|
|
177
|
Catabolite Repression
|
|
178
|
Catabolite Repression 2
|
Week 14
|
184
|
Gene Expression Is Controlled by Regulatory Proteins
|
|
185
|
Some Activators and Repressors Work by Allostery
|
|
186
|
Cooperative Binding and Allostery
|
|
187
|
Activators Recruit the Transcriptional Machinery
|
|
188
|
Activators Recruit Nucleosome Modifiers
|
|
189
|
Activators Recruit Additional Factors
|
|
190
|
Single integration & combinatorial control
|
|
191
|
Single integration & combinatorial control 2
|
|
192
|
Transcriptional Repressors
|
|
193
|
Signal Transduction Pathways
|
|
195
|
Plasmid Selection Systems
|
|
196
|
pUC Plasmid Cloning Vectors
|
|
197
|
Virus-based Cloning Vectors
|
|
198
|
? lambda Phage Vectors
|
|
201
|
Large Insert Capacity Cloning Vectors & YAC vectors
|
|
202
|
Vectors Used in Eukaryotic Cells
|
|
|
|