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BIO303 : Biochemistry II

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Course Info
Course Category	Biotechnology
Course Level	Undergraduate
Credit Hours	4
Pre-requisites	N/A
Instructor	Dr. Ammad Aslam Khan PhD Karolinska Institute

Course Contents
Sr. No. Title 1 BioEnergetics 2 The Standard Free-Energy 3 Eq constant and Standard free energy -I 4 Eq constant and Standard free energy -2 5 Chemical Logic and Common Biochemical Reactions-1 6 Chemical Logic and Common Biochemical Reactions -2 7 Chemical Logic and Common Biochemical Reactions- II-check final-3 8 Phosphoryl Group Transfers and ATP-.pptx 9 Phosphoryl Group Transfers in thioesters.pptx 10 Bioenergetics -3-Hydrolysis of ATP- part 1 11 Bioenergetics -3-Hydrolysis of ATP- part2.pptx 12 Bioenergetics -3-ATP energizes active transport and muscle contraction.pptx 13 Biological Oxidation -reduction reactions-2-4-18.pptx 14 Reduction potential - 15 Universal Electron Carriers 16 GLYCOLYSIS-1 17 Glycolysis- the pay off phase 18 Lecture - 18 - Preparatory phase of Glycolysis Requires ATP.pptx 19 Lecture - 19 - Preparatory phase of Glycolysis Requires ATP - cont-step 3 20 Preparatory phase of Glycolysis Requires ATP-3 21 Preparatory phase of Glycolysis -step 5 22 Pay off phase-Last steps of glycolysis-6-7 23 Lecture - 23 - Last steps of glycolysis. -8-10 24 Lecture - 24 - Energy generated during Glycolysis- part 1 and 2.pptx 25 Lecture - 25 - Importance of Phosphorylated Intermediates.pptx 26 Lecture - 26 - Fates of Pyruvate.pptx 27 Fates of Pyruvate -Lactic acid fermentation.pptx 28 Lecture - 28 - Ethanol Fermentation-.pptx 29 Uses of Fermentation in Industry.pptx 30 Industrial Fermentation-steps of fermentation.pptx 31 Industrial Fermentation-cont-5 32 Lecture - 32 - batch fermentation.pptx 33 Continuous Fermentation-6.pptx 34 Gluconeogenesis 35 Gluconeogenesis -cont 36 Gluconeogenesis - 1st by pass pathway-complete 37 Gluconeogenesis - 2nd-3rd by pass pathways- 38 Gluconeogenesis - cont - 4 39 Gluconeogenesis - cont -5 40 Pentose Phosphate Pathway - PPP -1 41 Pentose Phosphate Pathway - PPP -2-.pptx 42 Pentose Phosphate Pathway - PPP -3 43 feeder pathways of glycolysis -1. 44 feeder pathways of glycolysis -2 45 feeder pathways of glycolysis-3 - galactosemia 46 Denaturation of DNA 47 Denaturation of DNA.. contd 48 Renaturation of DNA 49 RNA 50 Difference b/w DNA and RNA 51 Structure of RNA 52 2ndry structure of RNA 53 Function of mRNA 54 Transfer RNA 55 Transfer RNa..1 56 Transfer RNa..2 57 Ribosomal RNa 58 snRNA 59 Enzymes 60 IUB Classification of enzymes- 61 Lyases 62 Ligases 63 Ligand 64 Mechanism of enzyme action 65 Mechanism of enzyme action..cont -1 66 Mechanism of enzyme action..cont -2 67 Mechanism of enzyme action..cont -3 68 Cofactor, coenzyme and prosthetic groups 69 Cofactor, coenzyme and prosthetic groups-1 70 Reaction rate and order of reaction 71 Factors affecting enzyme activity 72 Factors affecting enzyme activity (cont) 73 Enzyme kinetics 74 Enzyme kinetics..cont I 75 Enzyme kinetics..cont II 76 Enzymes...Michaelis-Mention Eq. 77 Enzymes...Michaelis-Mention Eq.cont-1 78 Enzymes...Michaelis-Mention Eq.cont-2 79 Enzymes...Michaelis-Mention Eq.cont-3 80 Enzymes... Order of Reaction 81 Lineweaver-burk plot 82 Inhibition of enzyme activity 83 Competitive inhibition 84 Competitive inhibition.. contd 85 Non-competitive inhibition 86 Enzymes.. effect on lineweaver-burk plot 87 Water 88 Ionization of water 89 Buffer System.. working solutions 90 Weak acids and bases 91 Water, pH and Buffer Solutions. 92 Buffer Solutions 93 Citric acid cycle-1 94 Citric acid cycle-2 95 Citric acid cycle-3 96 PDH complex-Citric acid cycle - 4 97 PDH complex-2 98 Steps of TCA-8 enzymes- 99 TCA-step 1. 100 Lesson - 100 - TCA-step2 101 TCA-step 4 102 Lesson - 102 - TCA-step 5 103 103 - TCA-ending.pptx 104 fatty acid metabolism-1 105 Lecture - 105 - Anaplerotic reactions of TCA- 106 Glyoxalate cycle -1 107 Glyoxalate cycle -2 108 Glyoxalate cycle -3 109 Fatty acid-1.pptx 110 fatty acid metabolism-2 111 fatty acid metabolism-effect of hormones- 112 carnitine shuttle 113 fatty acid metabolism-fatty acid oxidation-3 stages 114 Lesson - 114 - beta oxidation-complete 115 The four steps are repeated to yield acetyl CoA and ATP 116 "Oxidation of unsaturated fatty acids requires 2 additional steps " 117 Complete Oxidation of odd-numbered fatty acids requires 3 additional steps 118 Lecture - 118 - Coenzyme B12 119 Transcription Factors Turn on the Synthesis of Proteins for Lipid Catabolism 120 Oxidation in peroxisomes - 121 Oxidation in peroxisomes -cont 122 Omega Oxidation 123 Oxidation of phytanic acid 124 Ketone bodies 125 Ketone bodies-continued

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