Course Overview
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Course Synopsis
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The BIOCHEMSITRY-2 course mainly focuses on the metabolic aspects of four major building blocks of life e.g. The sugars, amino acids, fatty acids and nucelotides and the biosynthetic pathways which lead to synthesize the macromolecules from them e.g. Carbhohydrates, Proteins, Lipids and Nuceleic acids.In this all important course, we study the chemical reactions that provide the cell with both, the energy as well as raw materials necessary of life.
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Course Learning Outcomes
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Biochemistry-II
- Biochemistry-2 deals with study of the metabolism of major building blocks of life. The students who take this course will become familiar with the fundamental energy yielding and biosynthetic pathways i.e. Glycolysis, Krebs cycle, Pentose phosphate shunt, Gluconeogenesis and Urea cycle etc. Because all these pathways deals with the human physiology, the students of this course will also become familiar with important and critical metabolic disorders which arise because of malfunctioning of these pathways. In this way, this course is considered both as a basic as well as applied biochemistry course.
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Course Calendar
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Week 01
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2
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The Standard Free-Energy
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3
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Eq constant and Standard free energy -I
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4
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Eq constant and Standard free energy -2
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5
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Chemical Logic and Common Biochemical Reactions-1
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6
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Chemical Logic and Common Biochemical Reactions -2
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7
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Chemical Logic and Common Biochemical Reactions- II-check final-3
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8
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Phosphoryl Group Transfers and ATP-.pptx
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9
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Phosphoryl Group Transfers in thioesters.pptx
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Week 02
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10
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Bioenergetics -3-Hydrolysis of ATP- part 1
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11
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Bioenergetics -3-Hydrolysis of ATP- part2.pptx
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12
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Bioenergetics -3-ATP energizes active transport and muscle contraction.pptx
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13
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Biological Oxidation -reduction reactions-2-4-18.pptx
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15
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Universal Electron Carriers
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17
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Glycolysis- the pay off phase
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18
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Lecture - 18 - Preparatory phase of Glycolysis Requires ATP.pptx
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Week 03
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19
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Lecture - 19 - Preparatory phase of Glycolysis Requires ATP - cont-step 3
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20
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Preparatory phase of Glycolysis Requires ATP-3
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21
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Preparatory phase of Glycolysis -step 5
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22
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Pay off phase-Last steps of glycolysis-6-7
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23
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Lecture - 23 - Last steps of glycolysis. -8-10
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24
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Lecture - 24 - Energy generated during Glycolysis- part 1 and 2.pptx
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25
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Lecture - 25 - Importance of Phosphorylated Intermediates.pptx
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26
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Lecture - 26 - Fates of Pyruvate.pptx
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27
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Fates of Pyruvate -Lactic acid fermentation.pptx
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Week 04
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28
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Lecture - 28 - Ethanol Fermentation-.pptx
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29
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Uses of Fermentation in Industry.pptx
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30
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Industrial Fermentation-steps of fermentation.pptx
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31
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Industrial Fermentation-cont-5
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32
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Lecture - 32 - batch fermentation.pptx
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33
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Continuous Fermentation-6.pptx
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36
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Gluconeogenesis - 1st by pass pathway-complete
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Week 05
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37
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Gluconeogenesis - 2nd-3rd by pass pathways-
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38
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Gluconeogenesis - cont - 4
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39
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Gluconeogenesis - cont -5
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40
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Pentose Phosphate Pathway - PPP -1
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41
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Pentose Phosphate Pathway - PPP -2-.pptx
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42
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Pentose Phosphate Pathway - PPP -3
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43
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feeder pathways of glycolysis -1.
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44
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feeder pathways of glycolysis -2
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45
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feeder pathways of glycolysis-3 - galactosemia
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Week 06
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47
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Denaturation of DNA.. contd
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50
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Difference b/w DNA and RNA
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52
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2ndry structure of RNA
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Week 07
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60
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IUB Classification of enzymes-
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Week 08
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64
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Mechanism of enzyme action
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65
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Mechanism of enzyme action..cont -1
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66
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Mechanism of enzyme action..cont -2
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67
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Mechanism of enzyme action..cont -3
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68
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Cofactor, coenzyme and prosthetic groups
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69
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Cofactor, coenzyme and prosthetic groups-1
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70
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Reaction rate and order of reaction
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71
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Factors affecting enzyme activity
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72
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Factors affecting enzyme activity (cont)
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Week 09
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74
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Enzyme kinetics..cont I
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75
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Enzyme kinetics..cont II
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76
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Enzymes...Michaelis-Mention Eq.
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77
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Enzymes...Michaelis-Mention Eq.cont-1
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78
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Enzymes...Michaelis-Mention Eq.cont-2
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79
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Enzymes...Michaelis-Mention Eq.cont-3
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80
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Enzymes... Order of Reaction
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Week 10
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82
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Inhibition of enzyme activity
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83
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Competitive inhibition
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84
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Competitive inhibition.. contd
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85
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Non-competitive inhibition
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86
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Enzymes.. effect on lineweaver-burk plot
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89
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Buffer System.. working solutions
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Week 11
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91
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Water, pH and Buffer Solutions.
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96
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PDH complex-Citric acid cycle - 4
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Week 12
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98
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Steps of TCA-8 enzymes-
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100
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Lesson - 100 - TCA-step2
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102
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Lesson - 102 - TCA-step 5
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103
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103 - TCA-ending.pptx
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104
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fatty acid metabolism-1
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Week 13
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105
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Lecture - 105 - Anaplerotic reactions of TCA-
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110
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fatty acid metabolism-2
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Week 14
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111
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fatty acid metabolism-effect of hormones-
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113
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fatty acid metabolism-fatty acid oxidation-3 stages
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114
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Lesson - 114 - beta oxidation-complete
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115
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The four steps are repeated to yield acetyl CoA and ATP
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Week 15
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116
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"Oxidation of unsaturated fatty acids requires 2 additional steps "
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117
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Complete Oxidation of odd-numbered fatty acids requires 3 additional steps
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118
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Lecture - 118 - Coenzyme B12
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119
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Transcription Factors Turn on the Synthesis of Proteins for Lipid Catabolism
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120
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Oxidation in peroxisomes -
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Week 16
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121
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Oxidation in peroxisomes -cont
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123
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Oxidation of phytanic acid
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125
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Ketone bodies-continued
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