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BT102 : Microbiology

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Course Info

Course Category

 Biotechnology

Course Level

 Undergraduate

Credit Hours

 3

Pre-requisites

 N/A

Instructor

 Ms. Nafeesa Safdar
M.Phil Microbiology
Government College University Faisalabad

Course Contents

Microbiology and Microbes: An Introduction Classification of Microbes History of Microbiology History of Microbiology: Spontaneous generation theory Golden age of Microbiology Branches of Microbiology Microscopy Types of Microscope Staining Microbes Types of Staining 1_Gram Staining Types of Staining 2 _Acid fast and special staining Anatomy of Prokaryotes 1_ Eukaryotes Vs Prokaryotes Anatomy of Prokaryotes 2_Cell morphology Anatomy of Prokaryotes 3_Structures and their functions Anatomy of Prokaryotes 4_Flagella Anatomy of Prokaryotes 5_Axial filament, fimbriae and pili Anatomy of Prokaryotes 6_Cell wall Anatomy of Prokaryotes 6b_Gram positive Vs Gram negative bacterial cell walls Anatomy of Prokaryotes 7_ Atypical cell wall Anatomy of Prokaryotes 8_ Cell membrane Anatomy of Prokaryotes 9_ Movements across cell membrane Anatomy of Prokaryotes 10_ Cytoplasm Anatomy of Prokaryotes 11_Endospores Microbial Metabolism 1_Nutrient molecules and generation of ATP Microbial Metabolism 2_ Cellular respiration and fermentation Microbial Metabolism 3- Anaerobic respiration and nutritional classification of bacteria Microbial Growth Requirements 1_Temperature Microbial Growth Requirements 2_pH and osmotic pressure Microbial Growth Requirements 3_Elements Microbial Growth Requirements 4_Oxygen Microbial Growth Requirements 5_Culture Media Microbial Growth 6_Classification of culture media and culturing techniques Microbial Growth 7_Bacterial Growth Curve Microbial Growth 8_Estimation of Microbial Growth Microbial Control 1_Terminologies Microbial Control 2_Factors affecting Efficacy of Antibacterial Agents Microbial Control 3_Methods Microbial Control 4_Mechanical Methods Microbial Control 5_Gases Microbial Control 6_Chemical Methods Microbial Control 7_Chemicals Microbial Control 8_Physical Methods Microbial Control 9_Mechanisms Gene Transfer 1_Types Gene Transfer 2_Conjugation Gene Transfer 3_High Frequency Recombination Cells Gene Transfer 4_Transduction Classification I Microbial Identification 1- Methods Microbial Identification 2_Agglutination Tests Microbial Identification 3_ Precipitation Tests Microbial Identification 4_ELISA Microbial Identification 5_Western Blotting Microbial Identification 6_DNA probes & DNA Chips Microbial Identification 7_PCR Fungi 1_Introduction Fungi 2_Asexual spores Fungi 3_Sexual Spores Fungi 4_Yeast Fungi 5_Identification Fungi 6_Importance Lichen Algae 1_Introduction Algae 2_Types Virus 1_Introduction Virus 2_Replication Virus 3_Replication of Different Viruses Virus 4_Infection Virus 5_Cultivation Virus 6_Virusoids and Prions Epidemiology 1_Methods Epidemiology 2_Terminologies Epidemiology 3_Disease Epidemiology 4_Disease Transmission Pathogenicity 1_Mechanisms Pathogenicity 2_Evasion of Body Defenses Pathogenicity 3_Damage by Microbes Innate Immunity 1_Introduction Innate Immunity 2_Lines of Defense Innate Immunity 3_Cells and Secretions Adaptive Immunity 1_Introduction Adaptive Immunity 2_Antigens and Antibodies Adaptive Immunity 3_Clonal Selection Theory Adaptive Immunity 4_Role of Antibodies Adaptive Immunity 5_Cellular Immunity Adaptive Immunity 6_Immune Responses Special staining 1_Spore Special staining 2_Flagellar Special staining 3_Acid Fast Special staining 4_Capsular Lymphatics Hypersensitivity Type I Hypersensitivity Type II Hypersensitivity Type III Hypersensitivity Type IV Vaccine Antimicrobials 1_Discovery Antimicrobials 2_Scope and Spectrum Antimicrobials 3_Drug Targets Antimicrobials 4_Antibiotic Sensitivity Testing Antimicrobials 5_Modes of Action Antimicrobials 6_Antifungals and Antivirals Antimicrobials 7_Antiprotozoans and Antihelminthic Antimicrobials 8_Antibiotic Resistance Practical No.1 Biosafety levels Practical No. 2 Equipment and Laboratory Materials used in Microbiology Practical No. 3 Microscope and Microscopy Practical No. 4 Sterilization techniques (Physical/Heat method of sterilization) Practical No. 5 Sterilization techniques (Chemical method of sterilization) Practical No. 6 Sterilization techniques (Mechanical/Filtration methods) Practical No. 7 Types of media and culturing Practical No. 8 Culturing of bacteria in liquid medium Practical No. 9 Culturing of bacteria on solid medium Practical No.10 Colony and cell morphology Practical No. 11 Bacterial cell count Practical No. 12 Gram staining of bacteria Practical No.13 Antimicrobial Susceptibility Testing Practical No. 14 Biochemical tests Practical No. 15 Growth Kinetics
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