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BT301 : Introduction to Biotechnology

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Jahanzaib Azhar
MS Healthcare Biotechnology
NUST, Islamabad

Course Contents

Biotechnology- definition and history; Discovery of DNA; Structure and chemical composition of DNA; Organization of Genetic Material in Bacteria; Genome characterization-techniques; Foundations of biotechnology and interdisciplinary pursuit; Branches and applications of Biotechnology in medicine, agriculture (food, livestock, fisheries, algae, fungi, etc.); Benefits of Genomes research; Basic Techniques of Gene Manipulation; Restriction and modifying enzymes; cloning and expression vectors and their types; expression of recombinant proteins and their purification by affinity chromatography; Polymerase Chain Reaction (PCR) and its types; Genome mapping; Fluorescence in situ hybridization (FISH); DNA Microarray; Protection of biotechnological products; Safety in biotechnology; Public perception of Biotechnology; Biotechnology and ethics; Biotechnology and the developing world.