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BT301 : Introduction to Biotechnology

Course Overview

Course Synopsis

This course is developed to enable the students with fundamental concepts and techniques used in Biotechnology

Course Learning Outcomes

Biotechnology, Branches, Applications, Advances in Biotechnology, Scope of Biotechnology, Recombinant Techniques

  • Students will be able to learn about
  • The Biotechnology and its branches
  • Scope of Biotechnology
  • Techniques used in Biotechnology
  • Applications of Biotechnology Products
  • Molecules and Organisms used in Biotechnology Production

Course Calendar

Introduction to Biotechnology1PPT
Biotechnology in 21st Century2PPT
Roots of Biotechnology3PPT
Roots of Biotechnology (2)4PPT
Discovery of DNA5PPT
Dawn of Biotechnology6PPT
Commercialising Biotechnology7PPT
Biotechnology Giants8PPT
Nucleic Acids9PPT
Chemical composition of DNA10PPT
Nucleoside & Nucleotide11PPT
Types of Deoxyribonucleotides12PPT
How do Deoxyribonucleotides Join?13PPT
Structure of DNA14PPT
Work of Franklin & Wilkins (1950s)15PPT
Structure of DNA - 216PPT
Chemical composition of RNA17PPT
Types of Ribonucleotides18PPT
Types of RNAs19PPT
Structures of RNAs20PPT
Chemical composition of proteins21PPT
Primary structure of proteins22PPT
Secondary structure of proteins23PPT
Alpha Helix24PPT
Beta Pleated Sheets25PPT
Tertiary Structure of Proteins26PPT
Quaternary Structure of Proteins27PPT
Assignment No. 1
Genetic Materials in Viruses28PPT
Organization of Genetic Material in Bacteria -129PPT
Organization of Genetic Material in Bacteria - 230PPT
Organization of Genetic Material in Eukaryotes31PPT
Genetics and Genomics32PPT
Genomics, Proteomics and metabolomics33PPT
Why sequence genomes34PPT
Genome characterization-technique used35PPT
Genome analysis steps36PPT
Benefits of Genomes research37PPT
Genes and Sizes of Genomes38PPT
Viral Genomes39PPT
Bacterial genomes40PPT
Yeast genome41PPT
Mitochondrial genome42PPT
Chloroplast genome (cpDNA)43PPT
Eukaryotic genomes44PPT
Genomes Comparison45PPT
Comparing distantly and closely related species46PPT
Anatomy and organization of genome47PPT
Gene anatomy48PPT
Prokaryotic gene and eukaryotic gene49PPT
Types of eukaryotic DNA50PPT
Duplicated genes and Pseudo genes51PPT
Repetitive DNA52PPT
Mobile DNA53PPT
Movement of transposons and retrotransposons54PPT
Transposons in Prokaryotes and Eukaryotes55PPT
Long terminal repeats56PPT
Genetic Variations58PPT
Basic Techniques of Gene Manipulation-an overview59PPT
Isolation of genomic DNA60PPT
Agarose gel electrophoresis61PPT
Nucleic acid blotting62PPT
Southern blotting64PPT
Northern blotting65PPT
Western blotting66PPT
Transformation of E. coli , Electroporation67PPT
Transformation with other organisms68PPT
Polymerase Chain Reaction (PCR)69PPT
Cutting and Joining DNA molecules70PPT
Host-controlled restriction modification71PPT
Types of restriction and modification (R-M) system72PPT
Nomenclature of restriction endonucleases73PPT
Target sites of restriction endonucleases74PPT
Number and size of restriction fragments75PPT
Summary of restriction endonucleases76PPT
DNA modifying enzymes77PPT
Methods of joining DNA fragments78PPT
DNA ligase to create covalent recombinant DNA79PPT
Alkaline Phosphatase80PPT
Blunt end ligation via linker molecules81PPT
Homopolymer tailing83PPT
Cloning of cDNA by Homopolymer tailing84PPT
Interconversion of plasmid DNA86PPT
Effect of ethidium bromide on supercoiling of DNA87PPT
Phenotypic traits exhibited by plasmids88PPT
Properties of Conjugative and non-conjugative plasmids89PPT
Host range of plasmids90PPT
Partitioning and segregative stability of plasmids91PPT
Incompatibility of plasmids92PPT
Purification of plasmid DNA93PPT
Desirable properties of plasmid cloning vehicles94PPT
Natural plasmids as cloning vehicles95PPT
Grand Quiz (in lieu of Midterm Examination)
Use of pSC101 for cloning96PPT
pBR322, a purpose-built cloning vehicle97PPT
Cloning with pBR32298PPT
Improved vectors derived from pBR32299PPT
Runaway plasmid vectors100PPT
Bacteriophage lambda as cloning vector101PPT
Replication of phage-lambda DNA in lytic and lysogenic cycles102PPT
Modified lambda phages103PPT
Steps in cloning with lambda104PPT
Packaging phage-lambda DNA in vitro105PPT
Vectors for DNA sequencing: bacteriophage M13106PPT
Cosmid vectors107PPT
Modified schemes for cloning in Cosmid vectors108PPT
Phasmid vectors109PPT
Bacterial artificial chromosomes (BACs)110PPT
Yeast artificial chromosomes (YACs)111PPT
Shuttle and Expression vectors112PPT
Comparison of different cloning vectors-summary113PPT
Overview of cloning strategies114PPT
Genomic DNA libraries115PPT
Assignment No. 2
LambdaEMBL vectors for genomic library construction116PPT
Genomic libraries in high-capacity vectors117PPT
PCR as an alternative to genomic DNA cloning118PPT
Properties of cDNA119PPT
cDNA libraries120PPT
Preparation of cDNA for cloning121PPT
Improved methods for cDNA cloning122PPT
PCR as an alternative for cDNA cloning123PPT
Screening strategies124PPT
Screening by hybridization125PPT
Benton and Davis’ plaque lift procedure126PPT
Probe design127PPT
Chromosome walking128PPT
Chromosome jumping129PPT
Screening by PCR130PPT
Expression cloning131PPT
Immunochemical screening132PPT
Immunochemical screening of lambdagt11133PPT
South-western and north-western blotting134PPT
Screening by functional complementation135PPT
Requirement for expression in E. coli136PPT
Secretion of proteins137PPT
Protein trafficking138PPT
Stability of foreign proteins in E. coli139PPT
Constructing the optimal promoter140PPT
Optimizing translation initiation141PPT
Stability of mRNA and codon choice142PPT
The effect of plasmid copy number143PPT
Plasmid stability144PPT
Structural instability145PPT
Host cell physiology can affect the level of expression146PPT
DNA sequencing: Benefits and Applications147PPT
Maxam-Gilbert method148PPT
Chain termination or dideoxy procedure149PPT
Modifications of chain terminator sequencing150PPT
Automated DNA sequencing151PPT
Sequencing accuracy152PPT
DNA sequence databases153PPT
Cassette mutagenesis155PPT
Primer extension: the single primer method156PPT
PCR methods for site-directed mutagenesis157PPT
Basic PCR reaction158PPT
PCR principles and procedure159PPT
DNA polymerases160PPT
Degenerate primers162PPT
Types of PCR163PPT
Competitor RT-PCR164PPT
Real-time quantitative PCR (qPCR)165PPT
Nested PCR166PPT
Inverse PCR167PPT
Multiplex PCR168PPT
Applications of PCR172PPT
PCR-Forensic sciences174PPT
PCR-Agricultural sciences and environment175PPT
PCR-Molecular paleontology176PPT
Genome mapping177PPT
Markers for genome mapping178PPT
Genetic linkage mapping179PPT
Physical mapping180PPT
Physical versus linkage maps181PPT
The use of RFLPs in physical maps182PPT
STS in physical maps183PPT
SNPs as physical markers184PPT
Polymorphic DNA detection in the absence of sequence information185PPT
AFLPs detection in the absence of sequence information186PPT
Fluorescence in situ hybridization (FISH)187PPT
Radiation hybrid (RH) mapping188PPT
Happy mapping189PPT
Map integration190PPT
Sequencing Genome191PPT
High-throughput sequencing192PPT
Shotgun sequencing193PPT
Quiz No.1
Clone-by-clone sequencing194PPT
Orthologs and paralogs195PPT
Comparative genomics of bacteria196PPT
Comparative genomics of organelles197PPT
Comparative genomics of eukaryotes198PPT
DNA Microarray199PPT
Spotted DNA Arrays200PPT
Oligonucleotide Chips201PPT
Applications of Microarray-Microbial gene expression analysis202PPT
Applications of Microarray-Profiling in human disease203PPT
Phage Display204PPT
Screening phage display libraries205PPT
Applications of phage display206PPT
Knock Outs & Knock Inns207PPT
siRNA Technology208PPT
Applications of siRNA209PPT
Final-Term Exam
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