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BT735 : Advances in Fermentation Technology

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Course Info

Course Category

Biotechnology

Course Level

Graduate

Credit Hours

3

Pre-requisites

N/A

Instructor

Dr. Muhammad Mohsin Javed
PhD
Government College University, Lahore

Course Contents

Overview of fermentation technology (definition, types, ranges, applications of fermentation), Microbial growth kinetics (batch, fed-batch and continuous cultures), Design of bioreactors (basic function of a fermenter, aseptic operation and containment, body structure, construction material and construction of fermenter), temperature control system and design (cooling pipes and types of designs for temperature control), deigning agitation system (the impellers and types, stirrer glands and bearings, the mechanical seal, magnetic drives baffles), designing the aeration system (spargers and types porous sparger orifice sparger nozzle sparger combines sparger-agitator), valves and steam traps(valves and steam traps, gate valves, globe valves, piston valves, needle valves, plug valves, ball valves, butterfly valves, diaphragm valves, check valves and pressure control valves, sampling ports, feed ports), other fermentation vessels (the waldhof-type fermenter, the tower fermenter, airlift fermenters, the deep-jet fermenter, cyclone column, rotating disc fermenter), monitoring and control system (introduction and methods of measuring process variables), flow measurements and control pressure measurement and control (pressure reducing valves, pressure control valves, safety valves, steam traps), sensor probes, pH control and probes, temperature control, foam control, O2 and CO2 control (oxygen and CO2 electrode), aeration and agitation (the oxygen requirements oxygen supply oxygen demands KLa value determination), factors effecting KLa in fermentation vessel (the effect of air-flow, degree of agitation, medium rheology, microbial products, foam and antifoams, microbial biomass), the balance between oxygen supply and demand (controlling biomass concentration, controlling specific oxygen uptake rate), scale-up and scale down (factors of scale up, inoculums development, sterilization ,environmental parameters, scale up of aeration and agitation, regimes in stirred tank reactors, the scale up of air lift reactors), media for industrial fermentation  (introduction to media preparation requirements and types of media used for industrial fermenters), media formulation (water, energy source, carbon source, nitrogen source, minerals, growth factors, buffers, precursors, metabolic,  regulators, oxygen requirements and antifoams), medium optimization, sterilization modes  (media sterilization, sterilization of the fermenter, sterilization of the feed, sterilization of the liquid wastes, filter sterilization), design of batch sterilization, continuous sterilization process and design, product recovery (removal of microbial cells and solid matter, foam separation, precipitation, filtration, centrifugation, cell disruption, chromatographic techniques, drying, crystallization)