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CHE301 : Analytical Chemistry & Instrumentation

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Course Info

Course Category

Biotechnology

Course Level

Undergraduate

Credit Hours

3

Pre-requisites

N/A

Instructor

Dr.Yasmin
PhD, Posdoc
University of Sindh, Jamshoro

Course Contents

Course Contents:

1.     Introduction to Analytical Chemistry

1.1  Introduction to analytical chemistry

1.2  Methods used in analytical chemistry

1.3  Classical methods – chemical tests

1.4  Classical methods – flame tests

1.5  Classical methods – quantitative tests 

2.      CHROMATOGRAPHY

2.1  Introduction

2.2  Types of chromatography

2.3  Applications of chromatography 

3.      PAPER CHROMATOGRAPHY

3.1  Paper chromatography-introduction

3.2  Paper chromatography-technique

3.3  Types of paper chromatography

3.4  Applications of paper chromatography 

4.      THIN LAYER CHROMATOGRAPHY

4.1  Thin layer chromatography-introduction

4.2  Thin layer chromatography – technique

4.3  Applications of thin layer chromatography 

5.      GEL FILTRATION

5.1  Gel filtration-introduction

5.2  Gel filtration-factors

5.3  Gel filtration-technique part 1

5.4  Gel filtration technique-part 2

5.5  Gel filtration- applications 

6.      ION-EXCHANGE

6.1  Ion exchange chromatography - introduction

6.2  Ion exchange chromatography- technique

6.3  Ion exchange chromatography – types

6.4  Ion exchange chromatography-applications 

7.      AFFINITY CHROMATOGRAPHY

7.1  Affinity chromatography- introduction

7.2  Affinity chromatography-technique

7.3  Applications- affinity chromatography 

8.      HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

8.1  HPLC-introduction

8.2  HPLC- types

8.3  HPLC-technique

8.4  HPLC-applications 

9.      MASS SPECTROMETRY

9.1  Mass spectrometry-introduction

9.2  Mass spectrometry-technique

9.3  gas chromatography

9.4  GC technique

9.5  GC applications 

10.  GC-MS

10.1        GC-MS – Introduction

10.2        GC-MS-Instrumentation

10.3        GC-MS – Applications 

11.  LC-MS

11.1       LC-MS – Introduction

11.2       LC-MS-Instrumentation

11.3       LC-MS – Applications 

12.  SPECTROSCOPY

12.1        Spectroscopy-introduction

12.2        Spectroscopy- Technique 

13.  NUCLEAR MAGNETIC RESONANCE (NMR)

13.1        NMR-introduction

13.2        NMR-technique

13.3        NMR- applications 

14.  ULTRAVIOLET SPECTROSCOPY

14.1       UV- Vis Spectroscopy-introduction

14.2       UV- Vis Spectroscopy-terms

14.3       UV- Vis Spectroscopy- Principle

14.4       UV Vis spectroscopy-technique I

14.5       UV Vis spectroscopy-technique II

14.6       UV Vis spectroscopy-Spectrum analysis

14.7       UV – Vis Spectroscopy-applications 

15.  FLUORESCENCE SPECTROSCOPY

15.1       FLUORESCENCE-introduction

15.2       FLUORESCENCE-PRINCIPLE

15.3       FLUORESCENCE-technique

15.4       FLUORESCENCE-applications 

16.  ATOMIC ABSORPTION SPECTROSCOPY

16.1        Atomic absorption-introduction

16.2        Atomic absorption-technique

16.3        Atomic absorption-applications 

17.  ATOMIC EMISSION SPECTROSCOPY

17.1       Atomic emission-introduction

17.2       Atomic emission-principle

17.3       Atomic emission -technique

17.4       Atomic emission –applications

17.5       Atomic emission-types 

18.  INDUCTIVELY COUPLED PLASMA EMISSION SPECTROSCOPY (ICPMS)

18.1       ICPMS-introduction

18.2       ICPMS-principle

18.3       ICPMS-technique part 1

18.4       ICPMS-technique part 2

18.5       ICPMS-technique part 3

18.6        ICPMS-applications 

19.  FLOW CYTOMETRY

19.1       FLOW CYTOMETRY – introduction

19.2       FLOW CYTOMETRY-Principle

19.3       FLOW CYTOMETRY-technique-part 1

19.4       FLOW CYTOMETRY-technique part 2

19.5       FLOW CYTOMETRY-applications 

20.  X-RAY DIFFRACTION

20.1        X-Ray diffraction-introduction

20.2       X-Ray diffraction-principle

20.3       X-Ray diffraction- technique 1

20.4       X-Ray diffraction-technique part 2

20.5       X-Ray diffraction-DETECTORS

20.6       X-Ray diffraction-applications 

21.  General analytical instrumentations and methods of fractionation and characterization of proteins and nucleic acids

21.1        Introduction-fractionation and characterization of proteins

21.2        Dialysis-

21.3        Ultra-filtration –

21.4        Lyophilisation

21.5       Ultracentrifuge

21.6       Amino acid analyzer

Practical:

1.      Introduction to Analytical Chemistry

2.      Work Safety Instructions For Persons Working In Chemical Laboratory

3.      Laboratory Technique, Materials And Fundamental Operations

4.      Sample types and Analysis Methods

5.      Calculations Used in Analytical Chemistry

6.      Basic Tools of Analytical Chemistry

7.      Techniques for the separation and Purification of Biomolecules.

8.      Separation of biomolecules by Paper Chromatography

9.      Separation of biomolecules by Thin Layer Chromatography

10.  Determination of molecular weight of proteins by gel filtration

11.  Identification of sugars by UV/Visible spectrophotometer.

12.  Identification of proteins by UV/Visible spectrophotometer.

13.  Identification of electrolytes by UV/Visible spectrophotometer.

14.  Determination of sodium content in blood serum by flame photometer.

15.  Determination of potassium content in blood serum by flame photometer.

16.  Mineral analysis of plant tissues using atomic absorption spectrophotometer.