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ZOO731 : Advanced Analytical Techniques

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Course Info

Course Category

Biotechnology

Course Level

Graduate

Credit Hours

3

Pre-requisites

N/A

Instructor

Prof. Dr. Muhammad Arshad
Ph.D. (Molecular Immunology)
Medical University of Vienna, Austria

Course Contents

 Differential Staining Techniques, common biological stains, General staining procedure, Types of staining techniques, Gram staining, Haematoxylin and eosin staining, Acid fast Ziehl-Neelsen stain staining, Albert staining, Animal Preservation Techniques, Types of preservation techniques, Methods of preservation, Applications of animal preservation, Factors affecting preservation, Sampling techniques, What is a Sample, Sampling criteria, Sample Size, Purpose of sampling, Types of Sampling techniques, Polymerase chain reaction-introduction, Polymerase chain reaction- Principle, Polymerase chain reaction- components, Procedure, Types, Adnantages & Disadvantages, Applications, Microtomy-introduction, Types of microtomy, Methods (fixation and processing), Methods (Embedding), Method (sectioning and staining), Cryopreservation-introduction, Natural Cryopreservation, Cryopreservation agents, Effect of ice formation, Risks, Methods, Cryopreservation applications, Agarose Gel Electrophoresis- Introduction, Agarose Gel Electrophoresis- properties, Agarose Gel Electrophoresis- requirements,  Agarose Gel Electrophoresis- procedure, Agarose Gel Electrophoresis- Factors affecting AGE, Agarose Gel Electrophoresis- Applications, Northern Blotting- Introduction, Northern Blotting- procedure-I, Northern Blotting- procedure-II, Northern Blotting- procedure-III, Northern Blotting-Advantages and disadvantages, Northern Blotting-Reverse Northern blot, Northern Blotting-Applications, Western blotting-Introduction, Western Blotting-vs other blotting techniques, Western Blotting-components, Western Blotting-Procedure-I, Western Blotting-Procedure-II, Western Blotting-Procedure-III, Western Blotting-Procedure-Applications, SDS-Polyacrylamide Gel Electrophoresis- Introduction, SDS page Introduction, SDS-Polyacrylamide Gel Electrophoresis-Requirements, SDS-Polyacrylamide Gel Electrophoresis-procedure, SDS-Polyacrylamide Gel Electrophoresis- Factors, SDS-Polyacrylamide Gel Electrophoresis- Applications, Biosensors-Introduction, Biosensors-components, Biosensor- Principle, Biosensor- Basic Characteristics, Biosensors-Types-I, Biosensors-Types-II, Biosensor-Applications, DNA Barcoding-Introduction, DNA Barcode-A tool of genetic analysis, DNA Barcoding-Mitochondrial Genome, DNA Barcoding-Choice of Locus, DNA Barcoding-Procedure steps, DNA Barcoding-Applications, DNA Barcoding-Advantages and disadvantages, DNA Sequencing- Introduction, DNA Sequencing- Structure of DNA, DNA sequencing-Methods-I, DNA sequencing-Methods-II, DNA sequencing-Methods-III, DNA sequencing-Methods-IV, DNA Sequencing-Applications, Enzyme linked Immunosorbent Assay (ELISA)-Introduction, Enzyme linked Immunosorbent Assay (ELISA)-Principle, Enzyme linked Immunosorbent Assay (ELISA)-Types-1, Enzyme linked Immunosorbent Assay (ELISA)-Types-II, Enzyme linked Immunosorbent Assay (ELISA)-Types-III, Enzyme linked Immunosorbent Assay (ELISA)-Applications, Flow cytometry- Introduction, Flow cytometer- Principle, Flow cytometer- components, Flow Cytometry-Data analysis, Flow Cytometry-FACS, Flow Cytometry-Measurable parameters, Flow Cytometry-Applications, Protein Sequencing- Introduction, Protein Sequencing- Structure of proteins, Protein sequencing-Structural organization in proteins, Protein sequencing-Methods-1, Protein sequencing-Methods-II, Protein sequencing-Methods-III, Protein Sequencing-Applications, Southern Blotting-Introduction, Southern Blotting-Principle, Southern Blotting-Procedure-I, Southern Blotting-Procedure-II, Southern Blotting-Applications, Immunofluorescence- Introduction, Immunofluorescence- Principle, Immunofluorescence- TypeI, Immunofluorescence-Type II