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ZOO731 : Advanced Analytical Techniques

Course Overview
1 Differential Staining Techniques
2 Common biological stains
3 General staining procedure
4 Types of staining techniques
5 Gram staining
6 Haematoxylin and eosin (H&E) staining
7 Acid fast Ziehl-Neelsen stain staining
8 Albert’s staining
9 Animal Preservation Techniques
10 Types of preservation techniques
11 Methods of preservation
12 Applications of animal preservation
13 Factors affecting preservation
14 Sampling Techniques
15 What is a Sample
16 Sampling Criteria
17 Sample Size
18 Purpose of Sampling
19 Types of Sampling techniques
20 Polymerase chain reaction- Introduction
21 Polymerase chain reaction- Principle
22 Polymerase chain reaction- Components
23 Procedure
24 Types
25 Adnantages & Disadvantages
26 Applications
27 Microtomy-introduction
28 Types of microtomy
29 Methods (fixation and processing)
30 Methods (Embedding)
31 Method (sectioning and staining)
32 Cryopreservation-introduction
33 Natural Cryopreservation
34 cryopreservation agents
35 Effect of ice formation
36 Risks
37 Methods
38 Cryopreservation Applications
39 39-Agarose Gel Electrophoresis- Introduction
40 40-Agarose Gel Electrophoresis-Properties
41 41-Agarose Gel Electrophoresis-Requirements
42 42-Agarose Gel Electrophoresis- Procedure
43 43-Agarose Gel Electrophoresis- Factors affecting AGE
44 44-Agarose Gel Electrophoresis- Applications
45 45-Northern Blotting- Introduction
46 46-Northern Blotting-Procedure-I
47 47-Northern Blotting-Procedure-II
48 48-Northern Blotting-Procedure-III
49 49-Northern Blotting-Advantages and disadvantades
50 50-Northern Blotting-Reverse Northern Blot
51 51-Northern Blotting-Applications
52 Western Blotting- Introduction
53 Western Blotting-vs other blotting techniques
54 Western Blotting-Components
55 Western Blotting-Procedure-I
56 Western Blotting-Procedure-II
57 Western Blotting-Procedure-III
58 Western Blotting-Applications
59 SDS-Polyacrylamide Gel Electrophoresis- Introduction
60 SDS page introduction
61 SDS-Polyacrylamide Gel Electrophoresis-Requirements
62 SDS-Polyacrylamide Gel Electrophoresis- Procedure
63 SDS-Polyacrylamide Gel Electrophoresis- Factors
64 SDS-Polyacrylamide Gel Electrophoresis- Applications
65 Biosensors-Introduction
66 Biosensors-Components
67 Biosensor- Principle
68 Biosensors- Basic characteristics
69 Biosensors-Types-I
70 Biosensors-Types-II
71 Biosensor -Applications
72 DNA Barcoding-Introduction
73 DNA Barcode-A tool of genetic analysis
74 DNA Barcoding-Mitochondrial genome
75 DNA Barcoding-Choice of Locus
76 DNA Barcoding-ProcedureSteps
77 DNA Barcoding-Applications
78 DNA Barcoding-Advantages and disadvantages
79 DNA Sequencing- Introduction
80 DNA Sequencing-Structure of DNA
81 DNA sequencing-Methods-I
82 DNA Sequencing-Method-II
83 DNA Sequencing-Method-III
84 DNA Sequencing-Method-IV
85 DNA Sequencing-Applications
86 Enzyme linked Immunosorbent Assay (ELISA)-Introduction
87 Enzyme linked Immunosorbent Assay (ELISA)-Principle
88 Enzyme linked Immunosorbent Assay (ELISA)-Types-I
89 Enzyme linked Immunosorbent Assay (ELISA)-Types-II
90 Enzyme linked Immunosorbent Assay (ELISA)-Types-III
91 Enzyme linked Immunosorbent Assay (ELISA)-Applications
92 Flow Cytometry-Introduction
93 Flow cytometer- Principle
94 Flow Cytometry-Components
95 Flow Cytometry-Data analysis
96 Flow cytometry-FACS
97 Flow Cytometry-Measurable parameters
98 Flow Cytometry-Applications
99 Protein Sequencing- Introduction
100 Protein sequencing-Structure of proteins
101 Protein sequencing-Structural organization in proteins
102 Protein sequencing-Methods-I
103 Protein sequencing-Methods-II
104 Protein sequencing-Methods-III
105 Protein Sequencing-Applications
106 Southern Blotting- Introduction
107 Southern Blotting-Principle
108 Southern Blotting-Procedure-I
109 Southern Blotting-Procedure-II
110 Southern Blotting-Procedure-III
111 Southern Blotting-Applications
112 Immunofluorescence-Introduction
113 Immunofluorescence- Principle
114 Immunofluorescence-Type I
115 Immunofluorescence-Type II
Overview is not available for this course.
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