BIO201 : Cell Biology

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Course Info

Course Category

Molecular Biology

Course Level

Undergraduate

Credit Hours

4

Pre-requisites

N/A

Instructor

PROF. DR. FRIDOON JAWAD AHMAD
PhD
University of Wisconsin Madison

Course Contents

Introduction to Cell Biology Composition of Matter Molecules of Life Ptoteins: Polymers of Amino Acids Protein Structure Protein Interaction Carbohydrates: Sugar Polymers Carbohydrates: Glycosidic linkages Lipids: Nonpolar Hydrocarbons Lipids: Saturated & Unsaturated Nucleic Acids: DNA & RNA RNA & DNA Unit of Life Journey into the Cell Size Matters The Nucleus Ribosomes The Endoplasmic Reticulum Golgi & Lysosomes Mitochondria Cytoskeleton-1 Cytoskeleton-2 Motor Proteins The Miracle Workers Energy Free Energy Exergonic & Endergonic Reactions ATP Hydrolysis Enzymes Enzymes Mechanisms Enzyme Helpers Enzyme Regulation Membranes Membrane Components Recognition and Adhesion Transport across membranes Vesicle formation Cell division Cell Cycle Chromosome Mitosis Chromosomes-Conti... Meiosis-I Meiosis-II Meiosis-Conti.. Comparison-(Mitosis-Meiosis) Genetics introduction Mendel Experiment Learned lesson-Mendel Experiment Mendels first law of segregation Mendels second law Aberrant Phenomenna Linkage and recombination Genetics Maps Clinical Genetics-I Clinical genetics-I (Conti..) X linked disorders Multifactorial disorders Cytogenetic disorders Autosomal genetic disorders Cytogenetic disorders of sex chromosome Search for heredity molecules Search for heredity molecules 2 Investigating heredity molecules DNA function from structure Replication models DNA replication the molecular view DNA replication the process The Dilemma 1 The Dilemma 2 & 3 PCR technology PCR animation PCR Conti.. Detecting amplified DNA Gel electrophoresis animation PCR technology application DNA Fingerprinting DNA Sequencing Restriction Endonucleases Genetic Engineering RFLP DNA Transfer Genetic information is converted into proteins Central Dogma of Molecular Biology Transcription Genetic code Charging tRNA Translation workbench Translation Protein targeting Mutations Transcription regulation Transcription regulation in E.Coli Transcription Regulation in E.Coli 2 Transcription Regulation in Eukaryotes Eukaryotic Transcription RNA Processing Cell signalling and prehistoric world The Instructions and the Design The Modes and Principles The Modes and Principles II The Modes and Principles III Signalling in E.Coli G protein linked receptor Protein Kinases A G proteins G proteins vision Cell signalling and Us Cell signaling and memory Enzyme linked receptors Enzyme linked receptors 2 Enzyme linked receptors 2 (Conti...) RTK signalling gone wrong PI3 Kinase PKB apoptosis inhibition JAK-STAT signaling TGF-Beta receptor Family Proteolysis Signaling Pathways Immune system Immune system 2 Immune system 3 AIS The B-Cells The B-Cells 2 The Antibodies Immunological memory Acquired Immunological Tolerance Antibody diversity Class Switching T-Cells and TCR APCs activate T cells MHC Cytotoxic T cells Helper T Cells TH-1 and TH-2 TH-2 B-Cell activation Checks and Balances Complement System Complement System 2 The Alternative Pathway Neoplastic Transformation Growth promoting oncogenes Growth promoting oncogenes Cyclins and CDKs Growth promoting oncogenes Cyclins and CDKs (Conti..) DNA Repairing genes Apoptosis Genes Protein Sorting Protein Sorting Mechanism Nuclear Transport Nuclear localization signals [NLSs] and transport Nuclear Receptors The Ran Cycle Control of import and export Protein Transport into Mitochondria The Translocators ER Transport Introduction ER Transport Translocation Multipass Transmembrane Proteins Vesicle Transport COPI COPI the Retrieval Process Lysosomal Transport Endocytosis Tissue Replacement Organ repair via stem cell Customized stem cells Adult stem cells Cytokines induced stem cell proliferation and differentiation In-vitro organ/tissue fabrication Rules and cell culturing equipment Cell culturing equipment Getting started Mammalian cell culture Culturing Cells Sub culturing and cell banking Identifying different stages of cell cycle Practicals: practical 1 Microscopy and staining techniques Practical 2 Staining practical 3 Gram staining practical 3 Study of Prokaryotic cell practical 3 study of eukaryotic cell practical 4 Study of plastids practical 4 Study of chromoplast practical 5 Observation of chromoplast from rose petal practical 5 Study of chloroplast practical 6 study of mitosis practical 6 Study of meiosis practical 6 Smear/Squash preparation of Onion roots 1 practical 6 Smear/Squash preparation of Onion roots 2 practical 7 cheek cell examination under microscope 1 practical 7 human cheek cell examination under microscope 2 practical 6 A- Examination of onion cells practical 6 B Examination of onion skin epidermis cells practical 6 C Smear/Squash preparation of Onion roots 1