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BIO203 : Methods in Molecular Biology

Course Overview

Course Synopsis

It is an introductory course where students get an basic knowledge on techniques of Molecular Biology research.

Course Learning Outcomes

To acquaint students with the experimental aspects of molecular biology

  • To introduce with techniques used in recombinant DNA technology
  • To equip with molecular methods commonly used in the research and diagnostics

Course Calendar

Polymerase chain reaction1
Steps of PCR2
Ingredients of PCR3
Primers for PCR4
DNA polymerases5
Standard PCR reaction6
Interpretation of results7
Types of PCR8
Nested PCR9
Assignment No.1
Multiplex PCR10
Reverse transcriptase PCR11
Real time PCR12
Hot start PCR13
Asymmetric PCR14
Long PCR15
Allelic specific PCR16
Colony PCR17
In situ PCR18
Inverse PCR19
Assembly PCR21
Suicide PCR22
Methylation specific PCR23
Inter sequence specific PCR24
Ligation mediated PCR25
Grand Quiz in the place of Mid-Term Exam
Whole genome amplification PCR26
Mini primer PCR27
Advantages and limitations of PCR28
Applications of PCR29
Applications of PCR30
PCR-Forensic sciences32
PCR-Agricultural sciences and environment33
PCR-Molecular paleontology34
Types of restriction and modification (R-M) system35
Assignment No.2
Nomenclature of restriction endonucleases36
Target sites of restriction endonucleases37
Number and size of restriction fragments38
Summary of restriction endonucleases39
DNA modifying enzymes40
Methods of joining DNA fragments41
DNA ligase to create covalent recombinant DNA42
Alkaline Phosphatase43
Blunt end ligation via linker molecules44
Southern blotting46
Southern blotting procedure47
Southern blotting probes48
Transfer methods in southern blotting49
Mutations identification by southern blotting50
Northern blotting51
Northern blot applications52
Western blotting53
Western blotting procedure54
Final Term Exam
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