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BT503 : Environment Biotechnology

Course Overview

Course Synopsis

This course is designed to develop understanding of the students to the fundamental concepts of environmental biotechnology including a broad range of applications such as bioremediation, prevention, detection and monitoring, genetic engineering for sustainable development and better quality of living.This course will enable the students to get sufficient knowledge about the subject.

Course Learning Outcomes

Basic concept of environment biotechnology

  • Understand and comprehend the scope of Environment biotechnology.
  • Learn and apply different methods of pollution control
  • Classify and identify different pollutants
  • Understand basic techniques of waste removal
  • Students will learn basic concept of Genetic engineering, Bioremediation and Biomonitoring


Course Calendar

1 1.Introduction of Environmental Biotechnology
2 2.Role & Scope
3 3.The Global Environmental Market
4 4.Modalities and Local Influences (L04)
5 5.Using Biological Systems

6 6.Extremophiles
7 7.Thermophiles
8 8.Other Extremophiles
9 9.Xenobiotics and Other Problematic Chemicals
10 10.Endocrine Disruptors

11 11.Ongoing discoveries
12 12.Mobility of DNA
13 13.Genetic Manipulation
14 14.The Manipulation of Bacteria Without Genetic Engineering
15 15.The Manipulation of Bacteria by Genetic Engineering

16 16.Basic Principles of Genetic Engineering: Enzymes, solutions and equipment
17 17.Basic Principles of Genetic Engineering:Genomic Libraries
18 18.Basic Principles of Genetic Engineering DNA for transfer: cDNA libraries, & Polymerase chain reaction
19 19.Basic Principles of Genetic Engineering_Cloning Vectors
20 20.Basic Principles of Genetic Engineering_Expression Vectors and Reporter Genes
21 21.Basic Principles of Genetic Engineering Analysis of Recombinants
22 22.Basic Principles of Genetic Engineering Recombinant Bacteria & Yeast
23 23.Basic Principles of Genetic Engineering Recombinant Viruses
24 24.Transgenic Plants
25 25.Transformation of plants (Part I)
26 26.Transformation of plants (Part II)
27 27.Selected examples of developments in plant Genetic Engineering: Resistance against herbicides
28 28.Selected examples of developments in plant Genetic Engineering: Resistance against pests
29 29.Selected examples of developments in plant Genetic Engineering: Improved resistance to disease
30 30.Selected examples of developments in plant Genetic Engineering: Improved tolerance

31 31.Selected examples of developments in plant Genetic Engineering: Improved plants for phytoremediation
32 32.Selected examples of developments in plant Genetic Engineering: New products from plants
33 33.Introduction to Bio-indicators
34 34.Utilization of Bio-indicators
35 35.Types of bio-indicators: Plant Indicators
36 36 Types of bio indicators animal indicator
37 37 Types of bio indicators microbial indicator
38 38. Bio-monitoring
39 39. Plankton
40 40. Planktons as an indicator of water pollution
41 41.Phytoplankton
42 42.Evidences pertaining to phytoplankton
43 43. Zooplanktons
44 44. Evidences pertaining to zooplanktons
45 45.Aquatic insects as environmental indicators

46 46.Pollution and Pollution Control
47 47.Classifying Pollution
48 48.Toxicity
49 49.Persistence & Mobility
50 50.Ease of control, & Bio-accumulation
Assignment No.1-Inline
51 51.Chemistry of pollutants
52 52.The Pollution Environment
53 53.Pollution Control Strategies
54 54.Pollution Control Strategies air water & soil
55 55.Concentration and Containment
56 56.Practical Toxicity Issues (Part I)
57 57.Practical Toxicity Issues (Part II)
58 58.Industrial Pollution in Karachi
59 59.Practical Applications to Pollution Control.pptx
60 60. Biofilters

61 61. Biotrickling filters
62 62. Bioscrubbers
63 63. Other options (for treating gases)
64 64. Absorption, Adsorption, Incineration, & Ozonation
65 65. Main advantages of biotechnological approaches
Quiz No. 1
66 66. Clean’ Technology
67 67. Process Changes
68 68. Textile Industry
69 69. Leather industry
70 70. Desulphurization of coal and oil
71 71. Biological Control
72 72. Whole organism approaches (Part-I)
73 73. Whole organism approaches (Part-II)
74 74. Semiochemical agents (Part-I)
75 75. Semiochemical agents (Part II)
76 76. Bio-Substitutions
77 77. Barriers to uptake
78 78. Biomimetics
79 79. Simple bio-substitutions
80 80. Wastewater Treatment levels

81 81. Wastewater treatment (WWT) Operations, Process and System
82 82. Mechanisms for removal of pollutants in wastewater
83 83. Mechanisms for removal of pollutants from domestic sewage
84 84. Main Biological Wastewater Treatment Systems Stabilization Ponds
85 85. Main Biological Wastewater Treatment Systems Land Disposal
86 86. Main Biological Wastewater Treatment Systems Anaerobic System
87 87. Main Biological Wastewater Treatment Systems Activated sludge
88 88. Main Biological Wastewater Treatment Systems Aerobic Biofilm Reactors
89 89. Main Biological Wastewater Treatment Systems Preliminary Treatment
90 90. Main Biological Wastewater Treatment Systems Primary Treatment
91 91. Main Biological Wastewater Treatment Systems Secondary Treatment
92 92. Secondary Treatment Stabilisation ponds Facultative Ponds
93 93. Secondary Treatment Stabilisation ponds Anaerobic pond – facultative ponds systems
94 94. Secondary Treatment Stabilisation ponds Facultative aerated lagoon
95 95. Secondary Treatment Stabilisation ponds Complete-mix aerated lagoon – sedimentation pond systems
96 96. Secondary Treatment Stabilisation ponds High rate ponds
97 97. Secondary Treatment Stabilisation ponds Maturation ponds
98 98. Secondary Treatment Land disposal
99 99. Secondary Treatment land Disposal slow rate system
100 100. Secondary Treatment Land Disposal rapid infilteration
Assignment No.2-Inline

101 101. Secondary Treatment land disposal subsurface infilteration.
102 102. Secondary Treatment land diposal overland flow
103 103. Secondary Treatment constructed water land
104 104. Secondary Treatment constructed waterlands,sub-surface vertical horizontal.
105 105. Secondary Treatment anaerobic reactors septic tank
106 106. Secondary Treatment upflow anaerobic slug blanket
107 107. Secondary Treatment Upflow anaerobic sludge blanket reactors II
108 108. Secondary Treatment Activated Sludge System I
109 109. Secondary Treatment Activated Sludge System II
110 110. Secondary Treatment Conventional Activated Sludge I
111 111. Conventional Activated Sludge (II)
112 112. Activated Sludge System; Extended Aeration
113 113. Activated Sludge System; Intermittent operation
114 114. Activated sludge with biological nitrogen removal
115 115. Activated sludge with biological nitrogen and phosphorus removal
116 116. Aerobic biofilm reactors Low rate trickling filter
117 117. Aerobic biofilm reactors High rate trickling filter
118 118. Aerobic biofilm reactors Submerged aerated biofilters
119 119. Aerobic biofilm reactors Rotating biological contactors
120 120. Removal of Pathogenic Organisms
121 121. Analysis and Selection of the Wastewater Treatment Process, Criteria for Analysis
122 122. Comparison between the wastewater treatment systems
123 123. Sludge treatment & disposal (Introduction)
124 124. Relationships in sludge Relation between solid levels and water content
125 125. Sludge density Expression of the concentration of dry solids

126 126. Relation between flow, concentration and load
127 127. Quantity of Sludge Generated in the Wastewater treatment Processes
128 128. Sludge treatment stages
129 129. Sludge Thickening and Sludge Stabilization
130 130. Sludge Dewatering
131 131. Sludge Dewatering Sludge Drying Beds & Lagoons
132 132. Sludge Dewatering centrifuge
133 133. Sludge Disinfection Composting and Thermophilic aerobic digestion
134 134. Lime stabilization, Pasteurisation and Thermal treatment
135 135. Final Disposal of the Sludge
136 136. Contaminated Land and Bio-remediation
137 137. Remediation methods
138 138. In situ and Ex situ techniques
139 139. Intensive and Extensive Technologies
140 140. Bioremediation Documentary
Quiz No. 2
141 141. The Suitability of Bioremediation
142 142. Factors Affecting the Use of Bio-remediation
143 143. Biotechnology Selection
144 144. In situ Techniques
145 145. Site Monitoring for Biotechnological Applications
146 146. Ex situ Techniques
147 147. Process Selection and Integration
148 148. Case Studies
149 149. Oil Spill Bio-remediation
150 150. Phytotechnology and Photosynthesis

151 151. Terrestrial Phyto-Systems Metal Phytoremediation
152 152. Terrestrial Phyto-Systems Rhizofiltration
153 153. Terrestrial Phyto-Systems (TSP)
154 154. Phytoremediation – Plant Selection
155 155. Aquatic Phyto-Systems (APS) & Macrophyte Treatment System
156 156. APS Macrophyte Treatment System (MaTS) - II
157 157. Nutrient Film Techniques (NFTs) & Algal Treatment Systems (ATS)
158 158. Carbon sequestration by ATS
159 159. Pollution Detection
160 160. Case studies
GDB
161 161. Solid Waste Management
162 162. Sources and Types of Solid Waste
163 163. Initial Steps in Solid Waste Management
164 164. Key Components of Solid Waste Management
165 165. On-Site Disposal Options-I (For Solid Waste)
166 166. On-Site Disposal - II Options
167 167. Transportation Options
168 168. Off-Site Disposal Options
169 169. Intervention Levels
170 170. Protective Measures

171 171. Solid Waste Management
172 172. Integrated Environmental Biotechnology
173 173. Bioenergy
174 174. Derived Biofuels Methane biogas I
175 175. Derived Biofuels Methane Biogass II
176 176. Ethanol Fermentation
177 177. Short Rotation Coppicing I
178 178. Short Rotation Coppicing II
179 179. Biodiesel
180 180. The Great Biofuel Conundrum
181 181. Integrated Agricultural Applications & Plant Disease Suppression I
182 182. Integrated Agricultural Applications & Plant Disease Suppression II
183 183. Microbial Pesticides I
184 184. Microbial Pesticides II
185 185. Plant Microbe Interactions
186 186. Microbes External to the Plants
187 187. Microbes Internal to the Plants
188 188. Symbiotic Nitrogen Fixation
189 189. Endomycorrhizae and Plant Pathogens
190 190. Cauliflower Mosaic Virus

191 191. Case Study
192 192. Detoxification of Hazardous Chemicals I
193 193. Detoxification of Hazardous Chemicals II
194 194. Factors Causing Molecular Recalcitrance
195 195. Factors Causing Molecular Recalcitrance
196 196. Microorganisms Presence
197 197. Energy Metabolism Versus Cometabolism
198 198. Electron Donor Versus Electron Acceptors - I
199 199. Electron Donor Versus Electron Acceptors - II
200 200. Minimum Substrate Concentration - I
201 201. Minimum Substrate Concentration - II
202 202. Biodegradation of Problem Environmental Contaminants
203 203. Biodegradation of Problem Environmental Contaminants Pesticides I
204 204. Biodegradation of Problem Environmental Contaminants Pesticides II
205 205. Biodegradation of Problem Environmental Contaminants Pesticides III
206 206. Biodegradation of Problem Environmental Contaminants Hydrocarbons
207 207. Biodegradation of Problem Environmental Contaminants BTEX and MTBE I
208 208. Biodegradation of Problem Environmental Contaminants BTEX and MTBE II
209 209. Biodegradation of Problem Environmental Contaminants Polycyclic aromatic hydrocarbon I
210 210. Biodegradation of Problem Environmental Contaminants Polycyclic aromatic hydrocarbon II

211 211. Biodegradation of Problem Environmental Contaminants I
212 212. Biodegradation of Problem Environmental Contaminants II
213 213. Biodegradation of Problem Environmental Contaminants I
214 214. Biodegradation of Problem Environmental Contaminants II
215 215. Biodegradation of Problem Environmental Contaminants hycrocarbon I
216 216. Biodegradation of Problem Environmental Contaminants hydrocarbons II
217 217. Biodegradation of Problem Environmental Contaminants PCBs I
218 218. Biodegradation of Problem Environmental Contaminants PCBs II
219 219. Biodegradation of Problem Environmental Contaminants Pentachlorophenol
220 220. Biodegradation of Problem Environmental Contaminants Dioxin I
221 221. Biodegradation of Problem Environmental Contaminants Dioxins II
222 222. Biodegradation of Problem Environmental Contaminants Explosives
223 223. General Fate Modeling for Organic Chemicals - I
224 224. General Fate Modeling for Organic Chemicals - II
225 225. Inorganic Elements - I
226 226. Inorganic Elements - II
227 227. Inorganic Elements - III
228 228. Biosensors Introduction
229 229. Biosensors Configurations
230 230. Environmental Applications of Biosensors Toxicity
231 231. Endocrine Effect Biosensors
232 232. Biocides
233 233. Hormones
234 234. PCBs & Dioxins
235 235. Phenols