Course Overview
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Course Synopsis
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This course is designed to develop understanding of the students to the fundamental concepts of environmental biotechnology including a broad range of applications such as bioremediation, prevention, detection and monitoring, genetic engineering for sustainable development and better quality of living.This course will enable the students to get sufficient knowledge about the subject.
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Course Learning Outcomes
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Basic concept of environment biotechnology
- Understand and comprehend the scope of Environment biotechnology.
- Learn and apply different methods of pollution control
- Classify and identify different pollutants
- Understand basic techniques of waste removal
- Students will learn basic concept of Genetic engineering, Bioremediation and Biomonitoring
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Course Calendar
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Week 01
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1
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1.Introduction of Environmental Biotechnology
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3
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3.The Global Environmental Market
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4
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4.Modalities and Local Influences (L04)
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5
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5.Using Biological Systems
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9
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9.Xenobiotics and Other Problematic Chemicals
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10
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10.Endocrine Disruptors
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11
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11.Ongoing discoveries
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13
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13.Genetic Manipulation
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14
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14.The Manipulation of Bacteria Without Genetic Engineering
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15
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15.The Manipulation of Bacteria by Genetic Engineering
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Week 02
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16
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16.Basic Principles of Genetic Engineering: Enzymes, solutions and equipment
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17
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17.Basic Principles of Genetic Engineering:Genomic Libraries
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18
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18.Basic Principles of Genetic Engineering DNA for transfer: cDNA libraries, & Polymerase chain reaction
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19
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19.Basic Principles of Genetic Engineering_Cloning Vectors
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20
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20.Basic Principles of Genetic Engineering_Expression Vectors and Reporter Genes
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21
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21.Basic Principles of Genetic Engineering Analysis of Recombinants
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22
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22.Basic Principles of Genetic Engineering Recombinant Bacteria & Yeast
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23
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23.Basic Principles of Genetic Engineering Recombinant Viruses
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25
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25.Transformation of plants (Part I)
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26
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26.Transformation of plants (Part II)
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27
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27.Selected examples of developments in plant Genetic Engineering: Resistance against herbicides
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28
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28.Selected examples of developments in plant Genetic Engineering: Resistance against pests
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29
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29.Selected examples of developments in plant Genetic Engineering: Improved resistance to disease
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30
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30.Selected examples of developments in plant Genetic Engineering: Improved tolerance
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Week 03
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31
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31.Selected examples of developments in plant Genetic Engineering: Improved plants for phytoremediation
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32
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32.Selected examples of developments in plant Genetic Engineering: New products from plants
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33
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33.Introduction to Bio-indicators
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34
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34.Utilization of Bio-indicators
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35
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35.Types of bio-indicators: Plant Indicators
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36
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36 Types of bio indicators animal indicator
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37
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37 Types of bio indicators microbial indicator
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40
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40. Planktons as an indicator of water pollution
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42
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42.Evidences pertaining to phytoplankton
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44
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44. Evidences pertaining to zooplanktons
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45
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45.Aquatic insects as environmental indicators
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Week 04
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46
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46.Pollution and Pollution Control
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47
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47.Classifying Pollution
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49
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49.Persistence & Mobility
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50
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50.Ease of control, & Bio-accumulation
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51
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51.Chemistry of pollutants
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52
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52.The Pollution Environment
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53
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53.Pollution Control Strategies
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54
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54.Pollution Control Strategies air water & soil
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55
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55.Concentration and Containment
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56
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56.Practical Toxicity Issues (Part I)
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57
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57.Practical Toxicity Issues (Part II)
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58
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58.Industrial Pollution in Karachi
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59
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59.Practical Applications to Pollution Control.pptx
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Week 05
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61
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61. Biotrickling filters
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63
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63. Other options (for treating gases)
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64
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64. Absorption, Adsorption, Incineration, & Ozonation
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65
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65. Main advantages of biotechnological approaches
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70
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70. Desulphurization of coal and oil
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Assignment No.1
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71
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71. Biological Control
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72
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72. Whole organism approaches (Part-I)
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73
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73. Whole organism approaches (Part-II)
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74
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74. Semiochemical agents (Part-I)
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75
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75. Semiochemical agents (Part II)
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Week 06
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77
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77. Barriers to uptake
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79
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79. Simple bio-substitutions
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80
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80. Wastewater Treatment levels
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81
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81. Wastewater treatment (WWT) Operations, Process and System
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82
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82. Mechanisms for removal of pollutants in wastewater
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83
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83. Mechanisms for removal of pollutants from domestic sewage
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84
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84. Main Biological Wastewater Treatment Systems Stabilization Ponds
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85
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85. Main Biological Wastewater Treatment Systems Land Disposal
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86
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86. Main Biological Wastewater Treatment Systems Anaerobic System
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87
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87. Main Biological Wastewater Treatment Systems Activated sludge
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88
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88. Main Biological Wastewater Treatment Systems Aerobic Biofilm Reactors
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89
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89. Main Biological Wastewater Treatment Systems Preliminary Treatment
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90
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90. Main Biological Wastewater Treatment Systems Primary Treatment
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Week 07
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91
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91. Main Biological Wastewater Treatment Systems Secondary Treatment
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92
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92. Secondary Treatment Stabilisation ponds Facultative Ponds
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93
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93. Secondary Treatment Stabilisation ponds Anaerobic pond – facultative ponds systems
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94
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94. Secondary Treatment Stabilisation ponds Facultative aerated lagoon
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95
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95. Secondary Treatment Stabilisation ponds Complete-mix aerated lagoon – sedimentation pond systems
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96
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96. Secondary Treatment Stabilisation ponds High rate ponds
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97
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97. Secondary Treatment Stabilisation ponds Maturation ponds
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98
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98. Secondary Treatment Land disposal
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99
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99. Secondary Treatment land Disposal slow rate system
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100
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100. Secondary Treatment Land Disposal rapid infilteration
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Quiz No.1
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101
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101. Secondary Treatment land disposal subsurface infilteration.
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102
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102. Secondary Treatment land diposal overland flow
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103
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103. Secondary Treatment constructed water land
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104
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104. Secondary Treatment constructed waterlands,sub-surface vertical horizontal.
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105
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105. Secondary Treatment anaerobic reactors septic tank
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106
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106. Secondary Treatment upflow anaerobic slug blanket
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107
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107. Secondary Treatment Upflow anaerobic sludge blanket reactors II
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108
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108. Secondary Treatment Activated Sludge System I
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109
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109. Secondary Treatment Activated Sludge System II
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110
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110. Secondary Treatment Conventional Activated Sludge I
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Week 08
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111
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111. Conventional Activated Sludge (II)
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112
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112. Activated Sludge System; Extended Aeration
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113
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113. Activated Sludge System; Intermittent operation
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114
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114. Activated sludge with biological nitrogen removal
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115
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115. Activated sludge with biological nitrogen and phosphorus removal
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116
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116. Aerobic biofilm reactors Low rate trickling filter
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117
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117. Aerobic biofilm reactors High rate trickling filter
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118
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118. Aerobic biofilm reactors Submerged aerated biofilters
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119
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119. Aerobic biofilm reactors Rotating biological contactors
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120
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120. Removal of Pathogenic Organisms
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121
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121. Analysis and Selection of the Wastewater Treatment Process, Criteria for Analysis
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122
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122. Comparison between the wastewater treatment systems
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123
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123. Sludge treatment & disposal (Introduction)
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124
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124. Relationships in sludge Relation between solid levels and water content
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125
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125. Sludge density Expression of the concentration of dry solids
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126
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126. Relation between flow, concentration and load
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127
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127. Quantity of Sludge Generated in the Wastewater treatment Processes
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128
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128. Sludge treatment stages
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129
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129. Sludge Thickening and Sludge Stabilization
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130
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130. Sludge Dewatering
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Mid Semester Exam
Week 09
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131
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131. Sludge Dewatering Sludge Drying Beds & Lagoons
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132
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132. Sludge Dewatering centrifuge
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133
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133. Sludge Disinfection Composting and Thermophilic aerobic digestion
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134
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134. Lime stabilization, Pasteurisation and Thermal treatment
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135
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135. Final Disposal of the Sludge
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136
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136. Contaminated Land and Bio-remediation
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137
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137. Remediation methods
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138
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138. In situ and Ex situ techniques
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139
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139. Intensive and Extensive Technologies
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140
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140. Bioremediation Documentary
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141
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141. The Suitability of Bioremediation
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142
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142. Factors Affecting the Use of Bio-remediation
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143
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143. Biotechnology Selection
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144
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144. In situ Techniques
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145
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145. Site Monitoring for Biotechnological Applications
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146
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146. Ex situ Techniques
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147
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147. Process Selection and Integration
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149
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149. Oil Spill Bio-remediation
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150
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150. Phytotechnology and Photosynthesis
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Week 10
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151
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151. Terrestrial Phyto-Systems Metal Phytoremediation
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152
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152. Terrestrial Phyto-Systems Rhizofiltration
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153
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153. Terrestrial Phyto-Systems (TSP)
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154
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154. Phytoremediation – Plant Selection
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155
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155. Aquatic Phyto-Systems (APS) & Macrophyte Treatment System
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156
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156. APS Macrophyte Treatment System (MaTS) - II
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157
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157. Nutrient Film Techniques (NFTs) & Algal Treatment Systems (ATS)
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158
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158. Carbon sequestration by ATS
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159
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159. Pollution Detection
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Assignment No.2
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161
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161. Solid Waste Management
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162
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162. Sources and Types of Solid Waste
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163
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163. Initial Steps in Solid Waste Management
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164
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164. Key Components of Solid Waste Management
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165
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165. On-Site Disposal Options-I (For Solid Waste)
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166
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166. On-Site Disposal - II Options
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167
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167. Transportation Options
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168
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168. Off-Site Disposal Options
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169
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169. Intervention Levels
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170
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170. Protective Measures
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Week 11
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171
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171. Solid Waste Management
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172
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172. Integrated Environmental Biotechnology
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174
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174. Derived Biofuels Methane biogas I
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175
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175. Derived Biofuels Methane Biogass II
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GDB
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176
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176. Ethanol Fermentation
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177
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177. Short Rotation Coppicing I
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178
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178. Short Rotation Coppicing II
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180
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180. The Great Biofuel Conundrum
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181
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181. Integrated Agricultural Applications & Plant Disease Suppression I
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182
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182. Integrated Agricultural Applications & Plant Disease Suppression II
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183
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183. Microbial Pesticides I
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184
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184. Microbial Pesticides II
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185
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185. Plant Microbe Interactions
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186
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186. Microbes External to the Plants
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187
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187. Microbes Internal to the Plants
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188
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188. Symbiotic Nitrogen Fixation
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189
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189. Endomycorrhizae and Plant Pathogens
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190
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190. Cauliflower Mosaic Virus
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Quiz No.2
Week 12
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192
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192. Detoxification of Hazardous Chemicals I
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193
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193. Detoxification of Hazardous Chemicals II
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194
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194. Factors Causing Molecular Recalcitrance
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195
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195. Factors Causing Molecular Recalcitrance
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196
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196. Microorganisms Presence
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197
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197. Energy Metabolism Versus Cometabolism
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198
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198. Electron Donor Versus Electron Acceptors - I
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199
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199. Electron Donor Versus Electron Acceptors - II
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200
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200. Minimum Substrate Concentration - I
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201
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201. Minimum Substrate Concentration - II
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202
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202. Biodegradation of Problem Environmental Contaminants
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203
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203. Biodegradation of Problem Environmental Contaminants Pesticides I
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204
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204. Biodegradation of Problem Environmental Contaminants Pesticides II
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205
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205. Biodegradation of Problem Environmental Contaminants Pesticides III
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206
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206. Biodegradation of Problem Environmental Contaminants Hydrocarbons
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207
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207. Biodegradation of Problem Environmental Contaminants BTEX and MTBE I
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208
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208. Biodegradation of Problem Environmental Contaminants BTEX and MTBE II
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209
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209. Biodegradation of Problem Environmental Contaminants Polycyclic aromatic hydrocarbon I
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210
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210. Biodegradation of Problem Environmental Contaminants Polycyclic aromatic hydrocarbon II
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Week 13
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211
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211. Biodegradation of Problem Environmental Contaminants I
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212
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212. Biodegradation of Problem Environmental Contaminants II
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213
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213. Biodegradation of Problem Environmental Contaminants I
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214
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214. Biodegradation of Problem Environmental Contaminants II
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215
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215. Biodegradation of Problem Environmental Contaminants hycrocarbon I
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216
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216. Biodegradation of Problem Environmental Contaminants hydrocarbons II
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217
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217. Biodegradation of Problem Environmental Contaminants PCBs I
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218
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218. Biodegradation of Problem Environmental Contaminants PCBs II
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219
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219. Biodegradation of Problem Environmental Contaminants Pentachlorophenol
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220
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220. Biodegradation of Problem Environmental Contaminants Dioxin I
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221
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221. Biodegradation of Problem Environmental Contaminants Dioxins II
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222
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222. Biodegradation of Problem Environmental Contaminants Explosives
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223
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223. General Fate Modeling for Organic Chemicals - I
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224
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224. General Fate Modeling for Organic Chemicals - II
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225
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225. Inorganic Elements - I
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Week 14
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226
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226. Inorganic Elements - II
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227
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227. Inorganic Elements - III
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228
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228. Biosensors Introduction
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229
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229. Biosensors Configurations
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230
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230. Environmental Applications of Biosensors Toxicity
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231
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231. Endocrine Effect Biosensors
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