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GEN731 : Advanced Molecular Genetics

Course Overview

Course Synopsis

To prepare students with an advanced understanding of human genetic analysis, the techniques necessary to determine genotypes, and the ethical and social implications of determining that information.

Course Learning Outcomes

Advance knowlege of Molecular Biology and genetics

  • Understanding of genome. gene expression mechanisms and inheritance patterns, and to have the ability to critique and discuss ethical dilemmas relating to widely available genomic information.


Course Calendar

TopicLectureResourcePage
1Genetics DefinitionPPT
2Types of GeneticsPPT
3Molecules of LifePPT
4Ptoteins: Polymers of Amino AcidsPPT
5Protein StructurePPT
6Protein InteractionPPT
7Carbohydrates: Sugar PolymersPPT
8Carbohydrates: Glycosidic linkagesPPT
9Lipids: Nonpolar HydrocarbonsPPT
10Lipids: Saturated & UnsaturatedPPT
11Nucleic Acids: DNA & RNAPPT
12RNA & DNAPPT
13Clinical Genetics-IPPT
14Clinical genetics-I (Conti..)PPT
15X linked disordersPPT
16Multifactorial disordersPPT
17Cytogenetic disordersPPT
18Autosomal genetic disordersPPT
19Cytogenetic disorders of sex chromosomePPT
20Search for heredity moleculesPPT
21Search for heredity molecules 2PPT
22Investigating heredity moleculesPPT
23DNA function from structurePPT
24Replication modelsPPT
25DNA replication the molecular viewPPT
26DNA replication the processPPT
27The Dilemma 1PPT
28The Dilemma 2 & 3PPT
29PCR technologyPPT
30PCR animationPPT
31PCR Conti..PPT
32Detecting amplified DNAPPT
33Gel electrophoresis animationPPT
34PCR technology applicationPPT
35DNA FingerprintingPPT
36DNA SequencingPPT
37Restriction EndonucleasesPPT
38Genetic EngineeringPPT
39RFLPPPT
40DNA TransferPPT
41Genetic information is converted into proteinsPPT
42Central Dogma of Molecular BiologyPPT
43TranscriptionPPT
44Genetic codePPT
45Charging tRNAPPT
46Translation workbenchPPT
47TranslationPPT
48Protein targetingPPT
49MutationsPPT
50Transcription regulationPPT
51Transcription regulation in E.ColiPPT
52Transcription Regulation in E.Coli 2PPT
53Transcription Regulation in EukaryotesPPT
54Eukaryotic TranscriptionPPT
55RNA ProcessingPPT
56Cell signalling and prehistoric worldPPT
57The Instructions and the DesignPPT
58The Modes and PrinciplesPPT
59The Modes and Principles IIPPT
60The Modes and Principles IIIPPT
61Signalling in E.ColiPPT
62G protein linked receptorPPT
63Protein Kinases APPT
64G proteinsPPT
65G proteins visionPPT
66Cell signalling and UsPPT
67Cell signaling and memoryPPT
68Enzyme linked receptorsPPT
69Enzyme linked receptors 2PPT
70Enzyme linked receptors 2 (Conti...)PPT
71RTK signalling gone wrongPPT
72PI3 KinasePPT
73PKB apoptosis inhibitionPPT
74JAK-STAT signalingPPT
75TGF-Beta receptor FamilyPPT
76Proteolysis Signaling PathwaysPPT
77Multifactorial inheritancePPT
78Frequency of genetic disordersPPT
79Diagnostic tests for genetic disordersPPT
80Genetic counselingPPT
81Complications to basic inheritance patternPPT
82Drawing pedigreePPT
83Pedigree analysisPPT
84What is polymorphismPPT
85Types of polymorphismPPT
86Restriction fragment length polymorphismPPT
87VNTRPPT
88Short tandem repeat polymorphismPPT
89RAPD and AFLPPPT
90Distribution of SNPsPPT
91Screening and importance of SNPsPPT
92Genetic bases Problem 1PPT
93Genetic bases Problem 2PPT
94Genetic bases Problem 3PPT
95Genetic bases Problem 4PPT
96Genetic bases Problem 5PPT
97Genetic bases Problem 6PPT
98Genetic bases Problem 7PPT
99Genetic bases Problem 8PPT
100Genetic bases Problem 9PPT
101Genetic bases Problem 10PPT
102Genetic bases Problem 11PPT
103Genetic bases Problem 12PPT
104Genetic bases Problem 13PPT
105Genetic bases Problem 14PPT
106Genetic bases Problem 15PPT
107Genetic bases Problem 16PPT
108Genetic bases Problem 17PPT
109Source and recovery of DNAPPT
110DNA amount for molecular techniquesPPT
111Methods of DNA purificationPPT
112Organic method reagentsPPT
113Organic method summaryPPT
114Organic method procedure (I)PPT
115Organic method procedure (II)PPT
116Phenol chloroform in organic methodPPT
117Role of ethanol and isopropanolPPT
118Suspension and storage of DNAPPT
119Organic method propertiesPPT
120DNA extraction- non organic methodPPT
121Total cellular RNAPPT
122RNA purification- samplesPPT
123RNA purification -bloodPPT
124RNA purification- organic methodPPT
125RNA purification- affinity methodPPT
126RNA purification- human blood (I)PPT
127RNA purification - human blood (II)PPT
128RNA purification- human blood (III)PPT
129Quantitation of RNAPPT
130Chromosomes stainingPPT
131Giemsa StainingPPT
132Giemsa Staining procedurePPT
133C banding of chromosomesPPT
134Q banding of chromosomesPPT
135R banding of chromosomesPPT
136T banding of chromosomesPPT
137Polymerase chain reactionPPT
138Steps of PCRPPT
139Ingredients of PCRPPT
140Primers for PCRPPT
141DNA polymerasesPPT
142Standard PCR reactionPPT
143Interpretation of resultsPPT
144Types of PCRPPT
145Nested PCRPPT
146Multiplex PCRPPT
147Reverse transcriptase PCRPPT
148Real time PCRPPT
149Hot start PCRPPT
150Asymmetric PCRPPT
151Long PCRPPT
152Allelic specific PCRPPT
153Colony PCRPPT
154In situ PCRPPT
155Inverse PCRPPT
156AFLP PCRPPT
157Assembly PCRPPT
158Suicide PCRPPT
159Methylation specific PCRPPT
160Inter sequence specific PCRPPT
161Ligation mediated PCRPPT
162Whole genome amplification PCRPPT
163Mini primer PCRPPT
164Advantages and limitations of PCRPPT
165Applications of PCRPPT
166Southern blottingPPT
167Southern blotting procedurePPT
168Southern blotting probesPPT
169Transfer methods in southern blottingPPT
170Mutations identification by southern blottingPPT
171Northern blottingPPT
172Northern blot applicationsPPT
173Western blottingPPT
174Western blotting procedurePPT
 
 
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