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GEN731 : Advanced Molecular Genetics

Course Overview

Course Synopsis

To prepare students with an advanced understanding of human genetic analysis, the techniques necessary to determine genotypes, and the ethical and social implications of determining that information.

Course Learning Outcomes

Advance knowlege of Molecular Biology and genetics

  • Understanding of genome. gene expression mechanisms and inheritance patterns, and to have the ability to critique and discuss ethical dilemmas relating to widely available genomic information.

Course Calendar

1 Genetics Definition
2 Types of Genetics
3 Molecules of Life
4 Proteins: Polymers of Amino Acids
5 Protein Structure
6 Protein Interaction
7 Carbohydrates: Sugar Polymers
8 Carbohydrates: Glycosidic linkages
9 Lipids: Nonpolar Hydrocarbons
10 Lipids: Saturated & Unsaturated
11 Nucleic Acids: DNA & RNA
12 RNA & DNA
13 Clinical Genetics-I
14 Clinical genetics-I (Conti..)
15 X linked disorders
16 Multifactorial disorders
17 Cytogenetic disorders
18 Autosomal genetic disorders
19 Cytogenetic disorders of sex chromosome
20 Search for heredity molecules
21 Search for heredity molecules 2
22 Investigating heredity molecules
23 DNA function from structure
24 Replication models
25 DNA replication the molecular view
26 DNA replication the process
27 The Dilemma 1
28 The Dilemma 2 & 3
29 PCR technology
30 PCR animation
31 PCR Conti..
32 Detecting amplified DNA
33 Gel electrophoresis animation
34 PCR technology application
35 DNA Fingerprinting
36 DNA Sequencing
37 Restriction Endonucleases
38 Genetic Engineering
39 RFLP
40 DNA Transfer
41 Genetic information is converted into proteins
42 Central Dogma of Molecular Biology
43 Transcription
44 Genetic code
45 Charging tRNA
46 Translation workbench
47 Translation
48 Protein targeting
49 Mutations
50 Transcription regulation
51 Transcription regulation in E.Coli
52 Transcription Regulation in E.Coli 2
53 Transcription Regulation in Eukaryotes
54 Eukaryotic Transcription
55 RNA Processing
56 Cell signalling and prehistoric world
57 The Instructions and the Design
58 The Modes and Principles
59 The Modes and Principles II
60 The Modes and Principles III
61 Signalling in E.Coli
62 G protein linked receptor
63 Protein Kinases A
64 G proteins
65 G proteins vision
66 Cell signalling and Us
67 Cell signaling and memory
68 Enzyme linked receptors
69 Enzyme linked receptors 2
70 Enzyme linked receptors 2 (Conti...)
71 RTK signalling gone wrong
72 PI3 Kinase
73 PKB apoptosis inhibition
74 JAK-STAT signaling
75 TGF-Beta receptor Family
76 Proteolysis Signaling Pathways
77 Multifactorial inheritance
78 Frequency of genetic disorders
79 Diagnostic tests for genetic disorders
80 Genetic counseling
81 Complications to basic inheritance pattern
82 Drawing pedigree
83 Pedigree analysis
84 What is polymorphism
85 Types of polymorphism
86 Restriction fragment length polymorphism
87 VNTR
88 Short tandem repeat polymorphism
89 RAPD and AFLP
90 Distribution of SNPs
91 Screening and importance of SNPs
92 Genetic bases Problem 1
93 Genetic bases Problem 2
94 Genetic bases Problem 3
95 Genetic bases Problem 4
96 Genetic bases Problem 5
97 Genetic bases Problem 6
98 Genetic bases Problem 7
99 Genetic bases Problem 8
100 Genetic bases Problem 9
101 Genetic bases Problem 10
102 Genetic bases Problem 11
103 Genetic bases Problem 12
104 Genetic bases Problem 13
105 Genetic bases Problem 14
106 Genetic bases Problem 15
107 Genetic bases Problem 16
108 Genetic bases Problem 17
109 Source and recovery of DNA
110 DNA amount for molecular techniques
111 Methods of DNA purification
112 Organic method reagents
113 Organic method summary
114 Organic method procedure (I)
115 Organic method procedure (II)
116 Phenol chloroform in organic method
117 Role of ethanol and isopropanol
118 Suspension and storage of DNA
119 Organic method properties
120 DNA extraction- non organic method
121 Total cellular RNA
122 RNA purification- samples
123 RNA purification -blood
124 RNA purification- organic method
125 RNA purification- affinity method
126 RNA purification- human blood (I)
127 RNA purification - human blood (II)
128 RNA purification- human blood (III)
129 Quantitation of RNA
130 Chromosomes staining
131 Giemsa Staining
132 Giemsa Staining procedure
133 C banding of chromosomes
134 Q banding of chromosomes
135 R banding of chromosomes
136 T banding of chromosomes
137 Polymerase chain reaction
138 Steps of PCR
139 Ingredients of PCR
140 Primers for PCR
141 DNA polymerases
142 Standard PCR reaction
143 Interpretation of results
144 Types of PCR
145 Nested PCR
146 Multiplex PCR
147 Reverse transcriptase PCR
148 Real time PCR
149 Hot start PCR
150 Asymmetric PCR
151 Long PCR
152 Allelic specific PCR
153 Colony PCR
154 In situ PCR
155 Inverse PCR
156 AFLP PCR
157 Assembly PCR
158 Suicide PCR
159 Methylation specific PCR
160 Inter sequence specific PCR
161 Ligation mediated PCR
162 Whole genome amplification PCR
163 Mini primer PCR
164 Advantages and limitations of PCR
165 Applications of PCR
166 Southern blotting
167 Southern blotting procedure
168 Southern blotting probes
169 Transfer methods in southern blotting
170 Mutations identification by southern blotting
171 Northern blotting
172 Northern blot applications
173 Western blotting
174 Western blotting procedure
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