Course Overview
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Course Synopsis
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It is an introductory course where students get an basic knowledge on techniques of Molecular Biology research.
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Course Learning Outcomes
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The students will be able to:
- Understand different techniques used in Molecular Biology
- Analyze and differentiate different types of Polymerase chain reaction
- Equip with molecular methods commonly used in the research and diagnostics
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Course Calendar
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Week 01
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1
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Polymerase chain reaction
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Week 02
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7
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Interpretation of results
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11
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Reverse transcriptase PCR
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Week 03
Week 04
Week 05
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23
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Methylation specific PCR
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24
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Inter sequence specific PCR
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26
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Whole genome amplification PCR
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28
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Advantages and limitations of PCR
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Week 06
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33
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PCR-Agricultural sciences and environment
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34
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PCR-Molecular paleontology
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Week 07
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35
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Types of restriction and modification (R-M) system
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36
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Nomenclature of restriction endonucleases
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37
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Target sites of restriction endonucleases
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38
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Number and size of restriction fragments
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39
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Summary of restriction endonucleases
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Week 08
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41
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Methods of joining DNA fragments
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42
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DNA ligase to create covalent recombinant DNA
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44
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Blunt end ligation via linker molecules
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Week 09
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47
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Southern blotting procedure
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48
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Southern blotting probes
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49
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Transfer methods in southern blotting
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50
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Mutations identification by southern blotting
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Week 10
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52
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Northern blot applications
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54
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Western blotting procedure
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55
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55-Applications of western blotting
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56
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56- Difference between different blotting techniques
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Week 11
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57
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57- Single nucleotide polymorphism -1
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58
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58-Single nucleotide polyorphism -2
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59
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59- How to detect SNPs
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Week 12
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62
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62- DNA fingerprinting
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63
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63- DNA profiling process
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64
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64- Typs of DNA fingerprinting methods_1
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65
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65-Types of DNA fingerprinting methods -2
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66
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66-Applications of DNA fingerprinting
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67
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Protocol 1 Reagents preparation
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68
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Protocol 2 DNA extraction
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69
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protocol 3 Organic protocol
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70
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protocol 4 Inorganic protocol
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71
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Protocol 5 HOTSHOT Method of DNA extraction
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72
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Protocol 6. chelex method
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73
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protocol 7: silica absorption
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74
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Protocol 8. RNA extraction
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75
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Protocol 9. PRC and its types
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76
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Protocol 10 QPCR and probe designing
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78
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Protocol 12 SDS gel electrophoresis
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79
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Protocol 13 agarose gel electrophoresis
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81
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Protocol 15 Plasmid isolation
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82
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Protocol 16 phylogenetic analysis
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83
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techniques in molecular biology
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Week 13
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84
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Making Antibodies (Polyclonal Antibodies)
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86
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Freezing Cells and Cell Banking
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87
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Enzyme Immunosorbent Assays
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88
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Triple Antibody Sandwich ELISA
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89
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Double Antibody Sandwich ELISA
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Week 14
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91
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Immunofluorescent Microscopy
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92
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Immunosorbent Electron Microscopy
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93
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Electrophoresis of Proteins
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94
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Electrophoresis of Nucleic Acid
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96
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Ion Exchange Chromatography
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