BIO203 : Methods in Molecular Biology

Course Overview

Course Synopsis

It is an introductory course where students get an basic knowledge on techniques of Molecular Biology research.

Course Learning Outcomes

The students will be able to:

  • Understand different techniques used in Molecular Biology
  • Analyze and differentiate different types of Polymerase chain reaction
  • Equip with molecular methods commonly used in the research and diagnostics


Course Calendar

1 Polymerase chain reaction
2 Steps of PCR
3 Ingredients of PCR
4 Primers for PCR
5 DNA polymerases

6 Standard PCR reaction
7 Interpretation of results
8 Types of PCR
9 Nested PCR
10 Multiplex PCR
11 Reverse transcriptase PCR

12 Real time PCR
13 Hot start PCR
Assignment No. 1
14 Asymmetric PCR
15 Long PCR
16 Allelic specific PCR

17 Colony PCR
18 In situ PCR
19 Inverse PCR
20 AFLP PCR
21 Assembly PCR
22 Suicide PCR
Assignment No. 2

23 Methylation specific PCR
24 Inter sequence specific PCR
25 Ligation mediated PCR
26 Whole genome amplification PCR
27 Mini primer PCR
28 Advantages and limitations of PCR

29 Applications of PCR
30 Applications of PCR_2
31 PCR-Medicine
32 PCR-Forensic sciences
33 PCR-Agricultural sciences and environment
34 PCR-Molecular paleontology
Quiz No. 1

35 Types of restriction and modification (R-M) system
36 Nomenclature of restriction endonucleases
37 Target sites of restriction endonucleases
38 Number and size of restriction fragments
Quiz No. 2
39 Summary of restriction endonucleases
Mid Term

40 DNA modifying enzymes
41 Methods of joining DNA fragments
42 DNA ligase to create covalent recombinant DNA
43 Alkaline Phosphatase
44 Blunt end ligation via linker molecules
45 Adaptors

46 Southern blotting
47 Southern blotting procedure
48 Southern blotting probes
49 Transfer methods in southern blotting
50 Mutations identification by southern blotting

51 Northern blotting
52 Northern blot applications
53 Western blotting
54 Western blotting procedure
55 55-Applications of western blotting
56 56- Difference between different blotting techniques
GradedDiscussionBoard

57 57- Single nucleotide polymorphism -1
58 58-Single nucleotide polyorphism -2
59 59- How to detect SNPs
60 60- SNPs screening
61 61- SNPs haplotype
AssignmentNo3

62 62- DNA fingerprinting
63 63- DNA profiling process
64 64- Typs of DNA fingerprinting methods_1
65 65-Types of DNA fingerprinting methods -2
66 66-Applications of DNA fingerprinting
67 Protocol 1 Reagents preparation
68 Protocol 2 DNA extraction
69 protocol 3 Organic protocol
70 protocol 4 Inorganic protocol
71 Protocol 5 HOTSHOT Method of DNA extraction
72 Protocol 6. chelex method
73 protocol 7: silica absorption
74 Protocol 8. RNA extraction
75 Protocol 9. PRC and its types
76 Protocol 10 QPCR and probe designing
77 Protocol 11 RT PCR
78 Protocol 12 SDS gel electrophoresis
79 Protocol 13 agarose gel electrophoresis
80 Protocol 14 STR
81 Protocol 15 Plasmid isolation
82 Protocol 16 phylogenetic analysis
83 techniques in molecular biology

84 Making Antibodies (Polyclonal Antibodies)
85 Monoclonal Antibodies
86 Freezing Cells and Cell Banking
87 Enzyme Immunosorbent Assays
88 Triple Antibody Sandwich ELISA
89 Double Antibody Sandwich ELISA
Quiz No. 3

90 Microscopy
91 Immunofluorescent Microscopy
92 Immunosorbent Electron Microscopy
93 Electrophoresis of Proteins
94 Electrophoresis of Nucleic Acid
95 Chromatography
96 Ion Exchange Chromatography
Final Term