Course Overview
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Course Synopsis
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This course is designed for post graduate students who will explore basic concepts about molecular biology topics starting from nucleic acids DNA and RNA genes genome and then finally up to the gene expression level This course will enable the students to get sufficient knowledge about the subject to carry out research in the same field
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Course Learning Outcomes
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Understanding from central dogma of molecular biology to advance Concepts
- After the successful completion of this course students will develop basic knowledge and skills in molecular biology and will become aware with the complexity and harmony of the cell As students will proceed through the modules they will be able to apply this knowledge skill and awareness of the topics such as structure and functions of DNARNA and proteins DNARNA extraction PCR recombinant DNA technology blotting techniques concept of gene and chromosomes transcription translation and DNA replication in the field of Molecular Biology This course is aimed to provide ample knowledge to the students which is sufficient to fulfill the requirements of their degree An important objective is to provide a supportive environment for students to cultivate higher order thinking skills by synthesizing concepts and by analyzing and interpreting different questions
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Course Calendar
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Week 01
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1
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Introduction to Molecular Biology
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2
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History of Molecular Biology
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3
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Achievements of Molecular Biology
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5
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Chemical composition of DNA
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6
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Nucleoside & Nucleotide
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7
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Types of Deoxyribonucleotides
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8
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How do Deoxyribonucleotides Join?
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10
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Work of Franklin & Wilkins (1950s)
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12
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Chemical composition of RNA
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13
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Types of Ribonucleotides
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16
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Nature of Genetic Material
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17
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Griffith’s Experiments
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Week 02
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18
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Transformation Experiments
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19
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Avery, MacLeod, and McCarty’s experiment
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20
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Hershey & Chase experiment
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21
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Chemical composition of proteins
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22
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Primary structure of proteins
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23
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Secondary structure of proteins
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26
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Tertiary Structure of Proteins
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27
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Quaternary Structure of Proteins
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28
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Genetic Materials in Viruses
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29
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Organization of Genetic Material in Bacteria -1
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30
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Organization of Genetic Material in Bacteria - 2
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31
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Organization of Genetic Material in Eukaryotes
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Week 03
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37
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Experiment of Meselson & Stahl
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38
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Chemistry of DNA Synthesis - 1
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39
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Chemistry of DNA Synthesis - 2
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40
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Mechanism of DNA Polymerase
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41
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DNA Polymerases Resemble a Hand - 1
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42
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DNA Polymerases Resemble a Hand - 2
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47
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INITIATION OF REPLICATION
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50
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TYPE II TOPOISOMERASES
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Week 04
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56
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Cyclobutane pyrimidine dimer
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58
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x rays and gamma rays damage
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59
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DNA instability in water
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60
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water-mediated deamination
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61
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water mediated deamination 2
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62
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Oxidative damage to DNA
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63
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Oxidative damage to DNA (Conti..)
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64
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Alkylation damage to DNA
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65
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Alkylation damage to DNA (Conti..)
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67
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DNA damage by aflatoxins
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68
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Chemical cross-linking agents
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69
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DNA damage by Psoralen
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70
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DNA damage by Cisplatin
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71
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Base analogs and interclating agents
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72
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Direct reversal of DNA damage
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Week 05
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75
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Mechanism of CPD photolyase
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77
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Damage Reversal by Dealkylation
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80
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Base Excision Repair Pathway
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83
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Nucleotide Excision Repair
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84
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Nucleotide Excision Repair of UV-induced Damage
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85
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UvrA, UvrB, and UvrC Proteins
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88
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Mismatch Repair System in E. coli
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89
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MutS, MutL, & MutH Proteins
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90
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Mismatch Repair in Eukaryotes
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91
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Human Mismatch repair system
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Week 06
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93
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The Adapter Hypothesis of Crick
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94
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Discovery of the Transfer RNA
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95
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Discovery of the messenger RNA
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96
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Synthesis of RNA upon DNA Templates
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97
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Establishing the Genetic code
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100
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Transcription Process
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101
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Elongation during transcription
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102
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Transcription cycle in Bacteria
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103
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transition to the open step
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104
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transition to the open step 2
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105
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Initial transcription
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107
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the Elongating Polymerases
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108
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Termination of the Transcription
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109
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Termination of the Transcription II
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110
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Rho independent termination
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Week 07
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111
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Transcription in Eukaryotes
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112
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Formation of Pre-initiation Complex
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114
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General Transcription Factors
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115
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General Transcription Factors (Conti...)
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117
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RNA Elongation and Proofreading
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118
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RNA Elongation and Proofreading (Conti...)
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119
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Transcription Termination
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120
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Polymerases I and III
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121
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Polymerases I and III (Conti...)
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123
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How splicing site is determined
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127
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Self-Splicing Introns
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Week 08
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129
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Finding the Splice Sites Correctly
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130
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Cell cycle sys-presentation (1)
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131
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S-phase synthesis of DNA
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134
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chromosomal segregation and meiosis
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135
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Meiosis-Pairing and synaptonemal complex
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136
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Homologous recombination
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137
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Crossing-Over Is Highly Regulated
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138
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control of cell division
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139
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DNA damage blocks cell division
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140
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Limitations on number of cells.corr
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142
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Homologous recombination--
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144
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mitotic recombination
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147
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Transposones contain inverted ternminal repeats
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Week 09
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148
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Mechanism of transposition
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149
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Transposition in mAize
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150
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Transposition in mAize-2
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152
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Types of receptors in signal transduction
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153
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TYPES OF EXTRACELLULAR MESSENGERS
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154
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G protein coupled receptors
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155
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Receptor protein-tyrosine kinases
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156
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50- Phagocytes role in apoptosis_Formatted
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157
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signal transduction-1
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158
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signal transduction-2
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159
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Ending the G-protein mediated response
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160
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Types of second messenger
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161
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Types of second messenger-2
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162
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Extracellular messenger molecules
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163
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Protein-Tyrosine Kinases
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164
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Protein Kinases-activation
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165
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activation of downstream signaling pathways
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Week 10
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168
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Accessory proteins RAS-MAP
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169
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RAS-Map kinase cascade-final
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171
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Insulin receptor substrate 1 and 2
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172
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Glucose transport and diabetes
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173
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Role of calcium in cellular activities
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174
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Movement of Calcium ions
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176
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proteins activated by calcium
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177
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Why dying of cells is important
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178
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intracellular signals for apoptosis_Formatted
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179
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Extrinsic Pathway of apoptosis_Formatted
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180
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Mitochondrial pathway for apoptosis_Formatted
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181
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Inhibition of apoptosis_
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182
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Phagocytes role in apoptosis_Formatted
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183
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Introduction to epigenetics part 1
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184
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Introduction to epigenetics part 2
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Week 11
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185
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DNA methylation in healthy cells
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186
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DNA methylation pattern change in cancer cells
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187
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miRNA silencing in Human cancer
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188
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DNA methylation in tumor detection
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189
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Histone posttranslational modifications
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190
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Histone modifications in cancer
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191
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Epigenetics and its genetic syndromes
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193
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Role of transcription factors
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194
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DNA Sites Involved in Transcrption
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195
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Transcriptional activation role of enhancers, promotors and coactivators
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196
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Transcription repression
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197
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Processing level control
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198
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Cytoplasmic localization of mRNA
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199
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Control of mRNA translation
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200
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Control of mRNA stability
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201
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Role of microRNAs in translation_Formatted
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202
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Post-translational control protein stability_Formatted
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Week 12
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203
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DNA mocroarray and micro chips
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204
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whole chromosome transcriptional mapping
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207
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tissue specific functional profiling
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208
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locating target sites for transcription factors
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209
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Locating enhancers that bind unknown proteins_Formatted
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211
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in situ expression analysis
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212
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protein separation_Formatted
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213
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protein analysis_Formatted (1)
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215
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Proteins characterize different organisms and species
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216
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Protein interactions_Formattd
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217
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Bioinformatics-an overview_Formatted
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219
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How to access and use the online data_Formatted
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220
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making antibodies_Formatted
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Week 13
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221
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Monoclonol_formatting
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222
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freezing cells and cell banking_Formatted
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223
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antibodies to small molecules_Fomatted
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224
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95-Growing hybridomas_Formatted
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225
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Purification_Formatted
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226
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enzyme immunosorbent assays
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227
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tripple antibody sandwitch elisa_Formatted
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228
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double antibody sandwich_Formatting
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229
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enhanced elisa systems_Formatted
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230
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competitive elisa_Formatted
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231
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Immunoflorescent microscopy_Formatted
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232
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Immunosorbent electron microscopy_Formatted
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233
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epitope mapping_Formatted
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234
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Immunoblotting_Formatted
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236
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Immunoaffinity chromatography_Formatted
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237
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antibody based biosensors_Formatted
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Week 14
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239
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Lateral flow devices_Formatted
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241
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Analyzing protein complexes_Formatted
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242
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General principles_Formatted
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243
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mass analyzer_Foramatted
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244
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electrophoresis of proteins_Formatted
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245
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electro of nucleic acid
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246
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capillary electrophoresis
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247
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Microchip electrophoresis
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248
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High performance liquid chromatography
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249
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Adsorption chromatography
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250
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Partition chromatography
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251
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Ion exchange chromatography
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252
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Molecular size exclusion chromatography
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253
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Affinity chromatography
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255
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Support Media for electrophoresis
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