Course Overview
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Course Synopsis
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This course will help students to understand the building blocks of life "The cells", how they made up of, cell constituents, cell interaction, cell processes as well as it will explore basic concepts about molecular biology topics starting from nucleic acids (DNA and RNA), genes, genome and then finally up to the gene expression level.
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Course Learning Outcomes
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Learning this course will help students to get a through understanding of common cellular and molecular processes of body in relation to different body functions.
- This course will impart knowledge about the animal cell and its complex organization of architecture
- It will provide understanding about the unified role of a cell for the ultimate sustainability of the organisms.
- Enable students to understand various ultra-structural, molecular and functional aspects of the cells will be communicated in this course
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Course Calendar
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Week 01
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1
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Introduction to the Study of cell biology
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2
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Introduction to Molecular Biology
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3
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Introduction to Prokaryotic and Eukaryotic Cells
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9
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Introduction to Plasma Membrane
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10
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An overview of membrane functions
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Week 02
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11
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A brief history of studies on plasma membrane
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12
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Chemical composition of membranes
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13
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Membrane Lipids (Phosphoglycerides)
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14
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Membrane Lipids (Sphingolipids)
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15
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Membrane Lipids (Cholesterol)
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16
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Membrane Carbohydrates
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18
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Membrane lipids and membrane fluidity
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20
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Diffusion of Water through Membranes
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Week 03
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21
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The Diffusion of Ions through Membranes
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30
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Intermediate Filaments
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Week 04
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34
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Recognition and adhesion
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35
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Transport across membranes
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36
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The Endoplasmic Reticulum
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37
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ER Transport Introduction
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Week 05
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42
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Protein Transport into Mitochondria
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44
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Peroxisome & Glyoxisome
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49
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Nucleic Acids DNA & RNA
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Week 06
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51
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Chemical composition of DNA
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52
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Nucleoside & Nucleotide
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53
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Types of Deoxyribonucleotides
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54
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How do Deoxyribonucleotides Join
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57
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Chemical composition of RNA
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58
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Types of Ribonucleotides
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Week 07
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61
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Nature of Genetic Material
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62
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Organization of Genetic Material in Bacteria
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63
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Organization of Genetic Material in Bacteria 2
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64
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Organization of Genetic Material in Eukaryotes
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Week 08
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73
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Meiosis II (continuous…)
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74
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Comparison - (Mitosis- Meiosis)
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76
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Experiment of Meselson & Stahl
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77
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Chemistry of DNA Synthesis
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78
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Chemistry of DNA Synthesis 2
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79
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Mechanism of DNA Polymerase
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Week 09
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84
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INITIATION OF REPLICATION
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87
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TYPE II TOPOISOMERASES
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90
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Synthesis of RNA upon DNA Templates
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Week 10
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94
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Elongation during Transcription
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95
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Transcription Cycle in Bacteria
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96
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Transition to the Open Complex
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97
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Transition to the Open Complex 2
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99
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The Elongating Polymerase
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100
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Termination of Transcription
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Week 11
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101
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Termination of Transcription 2
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102
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Rho-independent Terminators
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103
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TRANSCRIPTION IN EUKARYOTES
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104
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Formation of pre-initiation complex
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106
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General Transcription Factors
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107
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General Transcription Factors 2
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109
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RNA Elongation and Proofreading
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110
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RNA Elongation and Proofreading 2
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Week 12
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111
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Transcription Termination
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113
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Polymerase I and III (continuous…)
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115
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How splicing site is determined
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119
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Self-Splicing Introns
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Week 13
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122
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Discovery of Messenger RNA
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126
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Discovery of Transfer RNA
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127
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Secondary structure of tRNA
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128
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Attachment of Amino Acids to tRNA
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130
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Structure of peptide bond
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Week 14
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131
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Binding Sites on Ribosomes for tRNA
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132
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Initiation of Translation
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133
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The Initiation Factors
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134
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Translation Elongation
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135
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The Ribosome Is a Ribozyme
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136
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Translocation in the Large Subunit
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137
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Termination of Translation
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138
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Termination of Translation 2
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139
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Nascent polypeptide processing & folding
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140
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Nascent polypeptide processing & folding 2
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141
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RNA processing (post translational modifications)
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142
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Establishing the Genetic Code
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145
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Practical 1 Study of Prokaryotic cell
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146
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Practical 2 Eukaryotic, Plant and Animal cells
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147
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Practical 3 Observation of wet mounts of human cheek cells employing bright and dark field microscopy
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148
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Practical 4 Study of cellular reproduction
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149
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Practical 5 Study of Mitosis: Smear/Squash preparation of Onion roots
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150
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Practical 6 Staining of Bacteria (Gram Staining)
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151
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Practical 7 DNA Extraction from Whole Blood 1
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152
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Practical 8 DNA Extraction from Whole Blood 2
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153
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Practical 9 DNA quantification by spectrophotometry using NanoDrop 1
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154
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Practical 10 DNA quantification by spectrophotometry using NanoDrop 2
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155
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Practical 11 Polymerase Chain Reaction (PCR) amplification 1
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156
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Practical 12 Polymerase Chain Reaction (PCR) amplification 2
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157
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Practical 13 Polymerase Chain Reaction (PCR) amplification 3
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158
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Practical 14 Agarose Gel Electrophoresis 1
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159
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Practical 15 Agarose Gel Electrophoresis 2
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160
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Practical 16 Agarose Gel Electrophoresis 3
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